Personal stethoscope disinfection practices and bacterial contamination: A cross-sectional study at the University Hospital Emergency Department in Belgrade, Serbia

Am J Infect Control. 2024 Feb;52(2):176-182. doi: 10.1016/j.ajic.2023.08.006. Epub 2023 Aug 19.

Abstract

Background: A significant reduction in bacterial growth on stethoscope membranes has been noticed after performing daily disinfection. Nevertheless, disinfection is rarely performed. We aimed to assess self-reported stethoscope disinfection practices among medical doctors, detect bacterial contamination on personal stethoscopes, and estimate the effectiveness of 70% ethanol as a stethoscope disinfecting agent.

Methods: To determine stethoscope disinfection practices, participants filled out a questionnaire (N = 47), followed by providing stethoscopes for bacterial analysis. Differences in bacterial contamination were observed through the self-reported frequency and method of stethoscope disinfection. The effect of disinfecting with 70% ethanol was evaluated by comparing the presence of bacterial growth before and after disinfection.

Results: The presence of bacterial growth was found in 78.7% of the stethoscope samples, with the median (interquartile range) number of colony-forming units at 25 (10-105). The frequency of disinfection greatly impacted the number of colony-forming units, and the method affected the presence of bacterial growth. Disinfection of stethoscope membranes using 70% ethanol resulted in a compelling 97.3% reduction of bacterial growth.

Conclusions: Adequate stethoscope disinfection is highly efficient in reducing bacterial contamination and as such should be considered a critical step in hygienic practices.

Keywords: Bacterial growth; Health care–associated infections; Stethoscope hygiene.

MeSH terms

  • 2-Propanol
  • Bacteria
  • Cross-Sectional Studies
  • Disinfection* / methods
  • Emergency Service, Hospital
  • Equipment Contamination
  • Ethanol
  • Hospitals
  • Humans
  • Serbia
  • Stethoscopes* / microbiology

Substances

  • 2-Propanol
  • Ethanol