Structural characterization and in vitro anti-colon cancer activity of a homogeneous polysaccharide from Agaricus bisporus

Ning Zhang; Yong Liu; Fang-Yuan Tang; Lin-Yuan Yang; Jun-Hui Wang

doi:10.1016/j.ijbiomac.2023.126410

Structural characterization and in vitro anti-colon cancer activity of a homogeneous polysaccharide from Agaricus bisporus

Int J Biol Macromol. 2023 Aug 18:251:126410. doi: 10.1016/j.ijbiomac.2023.126410. Online ahead of print.

Authors

Ning Zhang¹, Yong Liu², Fang-Yuan Tang¹, Lin-Yuan Yang¹, Jun-Hui Wang³

Affiliations

¹ The Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei 230009, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China.
² The Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei 230009, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China. Electronic address: liuyong@hfut.edu.cn.
³ The Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei 230009, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China. Electronic address: junhuiwang@hfut.edu.cn.

PMID: 37598827
DOI: 10.1016/j.ijbiomac.2023.126410

Abstract

Colon cancer is the third most prevalent cancer and the second most deadly cancer in the world. Anti-colon cancer activity of Agaricus bisporus polysaccharides has not been studied. In this paper, Agaricus bisporus polysaccharides were sequentially extracted by room temperature water, hot water, high pressure hot water, dilute alkaline solution and concentrated alkaline solution. A homogeneous polysaccharide (WAAP-1) was obtained using DEAE Cellulose-52 column. Physicochemical properties, structural characterization and anti-colon cancer activity of WAAP-1 were investigated. The results showed that WAAP-1 was a neutral polysaccharide with molecular weight of 10.1 kDa. The monosaccharide composition was glucose, mannose and galactose with a molar ratio of 84.95:8.97:4.50. The main chain was mainly composed of (1,4)-α-D-Glcp and (1,6)-β-D-Manp. In vitro anti-colon cancer results showed that WAAP-1 could significantly inhibit proliferation of colon cancer cell HT-29. It promoted apoptosis and inhibited epithelial mesenchymal transition of HT-29 by up-regulating the expression of Caspase-3, Bax and E-cadherin proteins and down-regulating the expression of Bcl-2 and Vimentin proteins. The results provided new potential possibilities for the development of novel functional foods or antitumor drugs.

Keywords: Agaricus bisporus; Anti-colon cancer activity; Homogeneous polysaccharide; Structural characterization; Structure-activity relationships.