Interleukin-8 involved in Nile Tilapia (Oreochromis niloticus) against bacterial infection

Xing Li; Baijian Jiang; Zhiqiang Zhang; Meiling Huang; Jiamin Feng; Yongxiong Huang; Kwaku Amoah; Yu Huang; Jichang Jian

doi:10.1016/j.fsi.2023.109004

Interleukin-8 involved in Nile Tilapia (Oreochromis niloticus) against bacterial infection

Fish Shellfish Immunol. 2023 Oct:141:109004. doi: 10.1016/j.fsi.2023.109004. Epub 2023 Aug 19.

Authors

Xing Li¹, Baijian Jiang¹, Zhiqiang Zhang¹, Meiling Huang¹, Jiamin Feng¹, Yongxiong Huang¹, Kwaku Amoah¹, Yu Huang², Jichang Jian³

Affiliations

¹ College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, China.
² College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China. Electronic address: huangyu@gdou.edu.cn.
³ College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China. Electronic address: jianjc@gdou.edu.cn.

PMID: 37598734
DOI: 10.1016/j.fsi.2023.109004

Abstract

Interleukin 8 (IL8) is vital in promoting inflammation and is a crucial mediator in various physiopathological processes while influencing immunological function. The effect of IL8 on the immunological response to acute bacterial infections in Nile tilapia (Oreochromis niloticus) remains unknown. This work found an IL8 gene from Nile tilapia (On-IL8). It includes a 285 bp open reading frame and codes for 94 amino acids. The transcript levels of On-IL8 were highest in the head-kidney tissue and sharply induced by Streptococcus agalactiae and Aeromonas hydrophila. Besides, in vitro experiments revealed that On-IL8 regulated a variety of immunological processes and promoted inflammatory responses. Moreover, On-IL8 suppressed the NF-κB signaling pathway, consistent with in vitro results. These significant findings serve as the basis for further investigation into how IL8 confers protection to bony fish in opposition to bacterial infections.

Keywords: Acute inflammation; Bacterial infection; Interleukin 8; Nile tilapia.

MeSH terms

Amino Acid Sequence
Animals
Cichlids*
Fish Diseases*
Fish Proteins / chemistry
Gene Expression Regulation
Interleukin-8 / genetics
Streptococcal Infections* / veterinary
Streptococcus agalactiae / physiology

Substances

Interleukin-8
Fish Proteins