Application of CRISPR/Cas12a in the rapid detection of pathogens

Yiheng Wang; Tianmu Yang; Guifang Liu; Longfei Xie; Jianying Guo; Wenguang Xiong

doi:10.1016/j.cca.2023.117520

Application of CRISPR/Cas12a in the rapid detection of pathogens

Clin Chim Acta. 2023 Aug 1:548:117520. doi: 10.1016/j.cca.2023.117520. Epub 2023 Aug 16.

Authors

Yiheng Wang¹, Tianmu Yang¹, Guifang Liu¹, Longfei Xie¹, Jianying Guo², Wenguang Xiong³

Affiliations

¹ Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
² College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: jyguo@scau.edu.cn.
³ Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: xiongwg@scau.edu.cn.

PMID: 37595863
DOI: 10.1016/j.cca.2023.117520

Abstract

The combination of clustered regularly interspaced short palindromic repeats (CRISPR) and its associated Cas protein is an effective gene-editing instrument. Among them, the CRISPR-Cas12a system forms a DNA-cleavage-capable complex with crRNA and exerts its trans-cleavage activity by recognising the PAM site on the target pathogen's gene. After amplifying the pathogenic gene, display materials such as fluorescent probes are added to the detection system, along with the advantages of rapid detection and high sensitivity of the CRISPR system, so that pathogenic bacteria can be diagnosed with greater speed and precision. This article reviews the mechanism of CRISPR-Cas12a in rapid detection, as well as its progress in the rapid detection of pathogenic bacteria in conjunction with various molecular biology techniques, in order to provide a foundation for the future development of a more effective detection platform.

Keywords: CRISPR/Cas12a; Diagnosis; Molecular biology amplification techniques; Rapid detection.

Publication types

Review

MeSH terms

CRISPR-Cas Systems* / genetics
Fluorescent Dyes*
Humans
RNA, Guide, CRISPR-Cas Systems

Substances

Fluorescent Dyes
RNA, Guide, CRISPR-Cas Systems