Effect of 10.6 μm laser moxibustion on inflammation in diabetic peripheral neuropathy rats

Yue Yin; Lina Wang; Ling Zhao; Lin Lin; Xueyong Shen

doi:10.3389/fendo.2023.1203677

Effect of 10.6 μm laser moxibustion on inflammation in diabetic peripheral neuropathy rats

Front Endocrinol (Lausanne). 2023 Aug 1:14:1203677. doi: 10.3389/fendo.2023.1203677. eCollection 2023.

Authors

Yue Yin¹, Lina Wang¹, Ling Zhao¹, Lin Lin², Xueyong Shen^{1

3}

Affiliations

¹ School of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
² School of Nursing, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
³ Laboratory of Systemic Physiology for Acupuncture and Meridian, Shanghai Research Center of Acupuncture and Meridian, Shanghai, China.

Abstract

Diabetic peripheral neuropathy (DPN) is the main cause of disability in diabetes patients but the efficacy of available drugs is poor. Moxibustion is an adjunctive treatment for DPN that can reduce symptoms. The peak value of the far infrared wavelength of 10.6 μm laser moxibustion is close to the infrared radiation spectrum of traditional moxibustion. Its effect is similar to that of moxibustion and does not cause pain, infection or produce irritating smoke. Twenty-four male SD rats were divided into control (Con), DPN, laser moxibustion (LM), and pyrrolidine dithiocarbamate (PDTC) groups (n=6/group). The DPN, LM and PDTC group rats were intraperitoneally injected with 1% streptozotocin (STZ) to induce a model of DPN. LM group rats were irradiated with a laser at bilateral ST36 acupoints for 15 min, once every other day, for 14 days. PDTC group rats were intraperitoneally injected with PDTC once a day. Body weight, blood glucose, and paw withdrawal mechanical threshold (PWMT) were measured and laser speckle imaging (LSI) performed before and after modeling and at 1 and 2 weeks after intervention. Two weeks after intervention, changes in serum interleukin 1β (IL1β), interleukin 6 (IL6), tumor necrosis factor α (TNFα) and nerve growth factor (NGF) were analyzed, and the abundance of NF-κB and IκB-α proteins and levels of NF-κB and IκB-α mRNAs in the sciatic nerve were observed. The results showed that 10.6 μm laser moxibustion can relieve pain, improve microcirculation, and alleviate inflammation in DPN rats, possibly via the NF-κB inflammatory pathway.

Keywords: NF- kappa B; diabetes mellitus; diabetic peripheral neuropathy; inflammation; laser moxibustion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diabetes Mellitus*
Diabetic Neuropathies* / therapy
Inflammation / therapy
Lasers
Male
Moxibustion*
NF-KappaB Inhibitor alpha
NF-kappa B
Rats
Rats, Sprague-Dawley

Substances

NF-kappa B
prolinedithiocarbamate
NF-KappaB Inhibitor alpha

Grants and funding

This study was funded by Shanghai Key Laboratory for Acupuncture Mechanism and Acupoint Function (21DZ2271800), Shanghai Sailing Program(21YF1447300), Scientificresearch project of Shanghai Health Committee (20204Y0489).