Highly-efficient synthesis of biogenic selenium nanoparticles by Bacillus paramycoides and their antibacterial and antioxidant activities

Pei Liu; Haiyu Long; Han Cheng; Mengdi Liang; Zhengwei Liu; Zhenlian Han; Zhen Guo; Hao Shi; Min Sun; Shuai He

doi:10.3389/fbioe.2023.1227619

Highly-efficient synthesis of biogenic selenium nanoparticles by Bacillus paramycoides and their antibacterial and antioxidant activities

Front Bioeng Biotechnol. 2023 Aug 1:11:1227619. doi: 10.3389/fbioe.2023.1227619. eCollection 2023.

Authors

Pei Liu^{1

2}, Haiyu Long^{1

2}, Han Cheng^{1

2}, Mengdi Liang^{1

2}, Zhengwei Liu^{1

2}, Zhenlian Han³, Zhen Guo^{1

2}, Hao Shi^{1

2}, Min Sun⁴, Shuai He^{1

2}

Affiliations

¹ Faculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, China.
² Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, China.
³ Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Science, Huaiyin Normal University, Huaian, China.
⁴ Huai'an Municipal Center for Disease Control and Prevention, Huaian, China.

Abstract

Introduction: Bacillus species are known for their ability to produce nanoparticles with various potential applications. Methods: In this study, we present a facile approach for the green synthesis of selenium nanoparticles (Se NPs) using the biogenic selenate-reducing bacterium Bacillus paramycoides 24522. We optimized the growth conditions and sodium selenite reduction efficiency (SSRE) of B. paramycoides 24522 using a response surface approach. Results: Se NPs were synthesized by reducing selenite ions with B. paramycoides 24522 at 37 °C, pH 6, and 140 r/min, resulting in stable red-colored Se NPs and maximal SSRE (99.12%). The synthesized Se NPs demonstrated lethality against Staphylococcus aureus and Escherichia coli with MICs of 400 and 600 μg/mL, and MBCs of 600 and 800 μg/mL, respectively, indicating the potential of Se NPs as antibacterial agents. Furthermore, the Se NPs showed promising antioxidant capabilities through scavenging DPPH radicals and reducing power. Discussion: This study highlights the environmentally friendly production of Se NPs using B. paramycoides 24522 and their possible applications in addressing selenium pollution, as well as in the fields of environment and biotechnology.

Keywords: Bacillus paramycoides; antibacterial activity; antioxidant activity; green synthesis; selenium nanoparticles.

Grants and funding

This work was supported by the National Natural Science Foundation of China (Grant numbers 32202158, 22278171); the Natural Science Foundation of Jiangsu Province of China (Grant number BK20220703); and the Agricultural Science and Technology Independent Innovation Project of Jiangsu Province, China [Grant number CX(22)3078].