Hemolysin from Aeromonas hydrophila enhances the host's serum enzyme activity and regulates transcriptional responses in the spleen of Cyprinus rubrofuscus

Mei Li; Dan Li; Fenglan Li; Wenli Liu; Shuang Wang; Gongqing Wu; Guofeng Wu; Guiliang Tan; Ziyi Zheng; Lin Li; Ziqiang Pan; Yiyao Liu

doi:10.1016/j.ecoenv.2023.115375

Hemolysin from Aeromonas hydrophila enhances the host's serum enzyme activity and regulates transcriptional responses in the spleen of Cyprinus rubrofuscus

Ecotoxicol Environ Saf. 2023 Sep 15:263:115375. doi: 10.1016/j.ecoenv.2023.115375. Epub 2023 Aug 15.

Authors

Mei Li¹, Dan Li², Fenglan Li³, Wenli Liu³, Shuang Wang⁴, Gongqing Wu⁴, Guofeng Wu³, Guiliang Tan³, Ziyi Zheng³, Lin Li³, Ziqiang Pan³, Yiyao Liu⁵

Affiliations

¹ School of Material Science and Food Engineering, University of Electronic Science and Technology of China Zhongshan Institute, Zhongshan 528402, China; School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610072, China; College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China. Electronic address: lm2570@163.com.
² School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610072, China.
³ School of Material Science and Food Engineering, University of Electronic Science and Technology of China Zhongshan Institute, Zhongshan 528402, China.
⁴ College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China.
⁵ School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610072, China; TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan, China. Electronic address: liuyiyao@uestc.edu.cn.

PMID: 37591129
DOI: 10.1016/j.ecoenv.2023.115375

Abstract

Aeromonas hydrophila is a conditional pathogen impacting public hygiene and safety. Hemolysin is a virulence factor of Aeromonas hydrophila that causes erythrocyte hemolysis, yet its transcriptional response to Cyprinus rubrofuscus remains unknown. Our investigation confirmed the hemolysis of hemolysin from A. hydrophila. Serum enzyme activity was evaluated weekly after C. rubrofuscus were immunized with hemolysin Ahh1. The results showed that the hemolysin enhances the serum superoxide dismutase (SOD), lysozyme (LZM), and catalase (CAT) activity, which reached a maximum on day 14. To elucidate the molecular interaction between hemolysin from A. hydrophila and the host, we performed transcriptome sequencing on the spleen of C. rubrofuscus 14 days post hemolysin infection. The total number of clean reads was 41.37 Gb, resulting in 79,832 unigenes with an N50 length of 1863 bp. There were 1982 significantly differentially expressed genes (DEGs), including 1083 upregulated genes and 899 downregulated genes. Transcript levels of the genes, such as LA6BL, CD2, and NLRC5, were significantly downregulated, while those of IL11, IL1R2, and IL8 were dramatically upregulated. The DEGs were mainly enriched in the immune disease, viral protein interaction with cytokine and cytokine receptor, and toll-like receptor pathways, suggesting that hemolysin stimulation can activate the transcriptional responses. RT-qPCR experiments results of seven genes, IL-8, STAT2, CTSK, PRF1, CXCL9, TLR5, and SACS, showed that their expression was highly concordant with RNA-seq data. We clarified for the first time the key genes and signaling pathways response to hemolysin from A. hydrophila, which offers strategies for treating and preventing diseases.

Keywords: Aeromonas hydrophila; Differentially expressed genes; Hemolysin; Koi carp (Cyprinus rubrofuscus); Transcriptome.

MeSH terms

Aeromonas hydrophila
Animals
Carps*
Hemolysin Proteins / genetics
Hemolysis
Spleen*

Substances

Hemolysin Proteins