A new ratiometric fluorescence nanosensor based on NaYF4:3%Er@NaYF4 upconversion nanoparticles for sensitive determination of Rose Bengal in water

Peiyao Wang; Silan Bai; Chen Chen; Yongtao You; Junhui Xiao; Xinrong Guo; Lishi Wang

doi:10.1016/j.saa.2023.123242

A new ratiometric fluorescence nanosensor based on NaYF₄:3%Er@NaYF₄ upconversion nanoparticles for sensitive determination of Rose Bengal in water

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15:303:123242. doi: 10.1016/j.saa.2023.123242. Epub 2023 Aug 10.

Authors

Peiyao Wang¹, Silan Bai¹, Chen Chen¹, Yongtao You¹, Junhui Xiao¹, Xinrong Guo², Lishi Wang³

Affiliations

¹ School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
² Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan 523808, People's Republic of China; Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, People's Republic of China. Electronic address: guoxinr@gdmu.edu.cn.
³ School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China. Electronic address: wanglsh@scut.edu.cn.

PMID: 37591018
DOI: 10.1016/j.saa.2023.123242

Abstract

Rose Bengal (RB) is used as a sensitizer in ambient water due to its property of catalyzing the production of singlet oxygen (¹O₂). However, this property also brings phototoxicity and carcinogenicity. The NaYF₄:3%Er@NaYF₄ core-shell upconversion nanoparticles (UCNPs) with higher upconversion efficiency was synthesized to detect RB in ambient water. Due to fluorescence resonance energy transfer (FRET) between RB and UCNPs, the upconversion fluorescence at 538 nm emitted by UCNPs was quenched by the RB, while the emission at 566 nm of RB raised. In the best conditions, the ratiometric emission intensity F₅₆₆/F₅₃₈ was positively proportional to RB concentration and the linear range was 0.04-15.0 μg·mL^-1 (R² = 0.996). The detection limit (S/N = 3) of RB was 2.46 ng·mL^-1. The recoveries ranged from 99.0% to 105.6% (relative standard deviation 0.97-3.24%, n = 3) in tap water and 100.3%-104.9% (relative standard deviation 0.66-1.94%, n = 3) in lake water. This proposed method exhibits lower detection limit and larger linear, which possesses practical application value to the detection of RB in water.

Keywords: Fluorescence resonance energy transfer; Lanthanide-doped upconversion nanoparticles; Rose Bengal.