Mobile Efficient Diagnostics of Infectious Diseases via On-Chip RT-qPCR: MEDIC-PCR

Kiran Shrestha; Seongryeong Kim; Jiyeon Han; Gabriela Morales Florez; Han Truong; Trung Hoang; Sajjan Parajuli; Tiara Am; Beomsoo Kim; Younsu Jung; Abdurhaman Teyib Abafogi; Yugyeong Lee; Seung Hyun Song; Jinkee Lee; Sungsu Park; Minhee Kang; Hee Jae Huh; Gyoujin Cho; Luke P Lee

doi:10.1002/advs.202302072

Mobile Efficient Diagnostics of Infectious Diseases via On-Chip RT-qPCR: MEDIC-PCR

Adv Sci (Weinh). 2023 Oct;10(28):e2302072. doi: 10.1002/advs.202302072. Epub 2023 Aug 16.

Authors

Kiran Shrestha^{1

2}, Seongryeong Kim^{1

2}, Jiyeon Han^{1

2}, Gabriela Morales Florez³, Han Truong^{1

2}, Trung Hoang¹, Sajjan Parajuli², Tiara Am^{1

4}, Beomsoo Kim⁵, Younsu Jung^{1

4}, Abdurhaman Teyib Abafogi⁶, Yugyeong Lee⁷, Seung Hyun Song⁸, Jinkee Lee^{1

6}, Sungsu Park^{1

6

7}, Minhee Kang^{9

10}, Hee Jae Huh¹¹, Gyoujin Cho^{1

4}, Luke P Lee^{1

12

13

14}

Affiliations

¹ Department of Biophysics, Institute of Quantum Biology, Sungkyunkwan University, Suwon, 16419, South Korea.
² Department of Intelligent Precision Healthcare Convergence, Sungkyunkwan University, Suwon, 16419, South Korea.
³ Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, 16419, South Korea.
⁴ Research Engineering Center for R2R Printed Flexible Computer, Sungkyunkwan University, Suwon, 16419, South Korea.
⁵ School of Electronic and Electrical Engineering, Sungkyunkwan University, Suwon, 16419, South Korea.
⁶ School of Mechanical Engineering, Sungkyunkwan University, Suwon, 16419, South Korea.
⁷ Department of Biomedical Engineering, Sungkyunkwan University, Suwon, 16419, South Korea.
⁸ Department of Electronics Engineering, Sookmyung Women's University, Seoul, 04310, South Korea.
⁹ Biomedical Engineering Research Center, Smart Healthcare Research Institute, Samsung Medical Center, Seoul, 06352, South Korea.
¹⁰ Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology), Sungkyunkwan University, Seoul, 06355, South Korea.
¹¹ School of Medicine, Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University, Seoul, 06351, South Korea.
¹² Harvard Medical School, Department of Medicine, Brigham Women's Hospital, Boston, MA, 02115, USA.
¹³ Department of Bioengineering, University of California at Berkeley, Berkeley, CA, 94720, USA.
¹⁴ Department of Electrical Engineering and Computer Science, University of California at Berkeley, Berkeley, CA, 94720, USA.

Abstract

The COVID-19 outbreak has caused public and global health crises. However, the lack of on-site fast, reliable, sensitive, and low-cost reverse transcription polymerase chain reaction (RT-PCR) testing limits early detection, timely isolation, and epidemic prevention and control. Here, the authors report a rapid mobile efficient diagnostics of infectious diseases via on-chip -RT-quantitative PCR (RT-qPCR): MEDIC-PCR. First, the authors use a roll-to-roll printing process to accomplish low-cost carbon-black-based disposable PCR chips that enable rapid LED-induced photothermal PCR cycles. The MEDIC-PCR can perform RT (3 min), and PCR (9 min) steps. Further, the cohort of 89 COVID-19 and 103 non-COVID-19 patients testing is completed by the MEDIC-PCR to show excellent diagnostic accuracy of 97%, sensitivity of 94%, and specificity of 98%. This MEDIC-PCR can contribute to the preventive global health in the face of a future pandemic.

Keywords: covid-19; diagnostics; low-cost; rapid; rt-qpcr.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

COVID-19 Testing
COVID-19* / diagnosis
Communicable Diseases* / diagnosis
Humans
Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity

Abstract

Publication types

MeSH terms

Grants and funding