Impact of clomazone on bacterial communities in two soils

Hairong He; Jiarui Huang; Zhenzhu Zhao; Weisheng Feng; Xiaoke Zheng; Pengqiang Du

doi:10.3389/fmicb.2023.1198808

Impact of clomazone on bacterial communities in two soils

Front Microbiol. 2023 Jul 31:14:1198808. doi: 10.3389/fmicb.2023.1198808. eCollection 2023.

Authors

Hairong He^#¹, Jiarui Huang^#¹, Zhenzhu Zhao¹, Weisheng Feng¹, Xiaoke Zheng¹, Pengqiang Du²

Affiliations

¹ College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China.
² College of Plant Protection, Henan Agricultural University, Zhengzhou, China.

^# Contributed equally.

Abstract

Introduction: Bacterial communities are important for soil functions, but the effect of clomazone on network complexity, composition, and stability is not well studied.

Method: In this study, two agricultural soils were used to test the impact of clomazone on bacterial communities, and the two soils were treated with three concentrations of clomazone (0, 0.8, 8, and 80 mg kg¹) in an incubator.

Results and discussion: Bacterial network nodes, links, and average degrees were all decreased by 9-384, 648-829, and 0.703-2.429, respectively. Based on keystone nodes, the topological roles of the nodes were also influenced by clomazone. Bacterial network composition was also impacted based on the analysis of similarity (ANOSIM) and network dissimilarity. Compared with control and clomazone treatments in both soils, the ANOSIM between control and all clomazone treatments was higher than 0.6, network dissimilarities were 0.97-0.98, shared nodes were 131-260, and shared links were 12-100. The bacterial network stability was decreased by clomazone, with decreased robustness by 0.01-0.016 and increased vulnerability by 0.00023-0.00147 in both soils. There were fewer bacterial network modules preserved after clomazone treatment, and the bacterial network community functions were also impacted in both soils. Based on these results, soil bacterial species connections, modularization, and network stability were significantly impacted by clomazone.

Keywords: bacterial network; clomazone; dissimilarity; network composition; stability.

Grants and funding

This research was funded by the National Natural Science Foundation of China (Grant Number 31901918) and the State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts (Grant Number 2010DS700124-KF2008).