Combined Use of Photobiomodulation and Curcumin-Loaded Iron Oxide Nanoparticles Significantly Improved Wound Healing in Diabetic Rats Compared to Either Treatment Alone

Manouchehr Hekmat; Houssein Ahmadi; Farazad Baniasadi; Behnaz Ashtari; Parvaneh Naserzadeh; Mansooreh Mirzaei; Hamidreza Omidi; Atarodalsadat Mostafavinia; Abdollah Amini; Michael R Hamblin; Sufan Chien; Mohammad Bayat

doi:10.34172/jlms.2023.18

Combined Use of Photobiomodulation and Curcumin-Loaded Iron Oxide Nanoparticles Significantly Improved Wound Healing in Diabetic Rats Compared to Either Treatment Alone

J Lasers Med Sci. 2023 Jun 12:14:e18. doi: 10.34172/jlms.2023.18. eCollection 2023.

Authors

Affiliations

¹ Department of Cardiac Surgery, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
² Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
³ School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
⁴ Department of Medical Nanotechnology. Faculty of Advanced Technologies in Medicine Iran University of Medical Sciences. Tehran, Iran.
⁵ Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
⁶ Department of Anatomical Sciences & Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
⁷ Laser Research Centre, Faculty of Health Science, University of Johannesburg, Doornfontein, South Africa, and Radiation Biology Research Center, Iran University of Medical Sciences, Tehran, Iran.
⁸ Price Institute of Surgical Research, University of Louisville, and Noveratech LLC, Louisville, KY, USA.

Abstract

Introduction: Here, we assess the therapeutic effects of photobiomodulation (PBM) and curcumin (CUR)-loaded superparamagnetic iron oxide nanoparticles (SPIONs), alone or together, on the maturation step of a type 1 diabetes (DM1) rat wound model. Methods: Full-thickness wounds were inflicted in 36 rats with diabetes mellitus (DM) induced by the administration of streptozotocin (STZ). The rats were randomly allocated to four groups. Group one was untreated (control); group two received CUR; group 3 received PBM (890 nm, 80 Hz, 0.2 J/cm²); group 4 received a combination of PBM plus CUR. On days 0, 4, 7, and 15, we measured microbial flora, wound closure fraction, tensile strength, and stereological analysis. Results: All treatment groups showed a substantial escalation in the wound closure rate, a substantial reduction in the count of methicillin-resistant Staphylococcus aureus (MRSA), a substantial improvement in wound strength, a substantially improvement in stereological parameters compared to the control group, however, the PBM+CUR group was superior to the other treatment groups (all, P≤0.05). Conclusion: All treatment groups showed significantly improved wound healing in the DM1 rat model. However, the PBM+CUR group was superior to the other treatment groups and the control group in terms of wound strength and stereological parameters.

Keywords: Diabetes mellitus; Low level laser therapy; Magnetic iron oxide nanoparticles; Photobiomodulation; Wound healing.