Gossypium hirsutum gene of unknown function Gohir.A03G0737001 encodes a potential Chaperone-like Protein of protochlorophyllide oxidoreductase (CPP1)

Alana N Osborne; Andrew Osagiede; Amanda R Storm; Amanda M Hulse-Kemp; Angela K Stoeckman

doi:10.17912/micropub.biology.000867

Gossypium hirsutum gene of unknown function Gohir.A03G0737001 encodes a potential Chaperone-like Protein of protochlorophyllide oxidoreductase (CPP1)

MicroPubl Biol. 2023 Jul 30:2023:10.17912/micropub.biology.000867. doi: 10.17912/micropub.biology.000867. eCollection 2023.

Authors

Alana N Osborne¹, Andrew Osagiede¹, Amanda R Storm², Amanda M Hulse-Kemp^{3

4}, Angela K Stoeckman⁵

Affiliations

¹ Chemistry, Bethel University, Saint Paul, Minnesota, United States.
² Biology, Western Carolina University, Cullowhee, NC.
³ Genomics and Bioinformatics Research Unit, USDA-ARS, Raleigh, NC.
⁴ Department of Crop and Soil Sciences, North Carolina State University, Raleigh, North Carolina, United States.
⁵ Chemistry Department, Bethel University, Saint Paul, Minnesota, United States.

Abstract

A gene of unknown function identified in Gossypium hirsutum , Gohir.A03G0737001.1, was studied using sequence and bioinformatic tools. The encoded protein (referred to here as GhCPP1-A0A1U8HKT6) was predicted to function as a Chaperone-like protein of protochlorophyllide oxidoreductase (CPP1), which is involved with initiation of photochemical reactions of chlorophyll biosynthesis. Sequence analysis indicates it is embedded in the chloroplast envelope membrane through four transmembrane regions and contains a J-like domain that is structurally similar to the J domain of DnaJ/Hsp40 "holdase" chaperone proteins.

Grants and funding

This research was funded in part by the U.S. Department of Agriculture Agricultural Research Service (USDA-ARS) project number 6066-21310-005-00D and Cotton Incorporated project 18-274 to AMH-K. We have no additional external funding to report.