Acylhydrazone-derived whole pectin-based hydrogel as an injectable drug delivery system

Shu-Ya Wang; Maryamgul Tohti; Jia-Qi Zhang; Jun Li; De-Qiang Li

doi:10.1016/j.ijbiomac.2023.126276

Acylhydrazone-derived whole pectin-based hydrogel as an injectable drug delivery system

Int J Biol Macromol. 2023 Aug 13:251:126276. doi: 10.1016/j.ijbiomac.2023.126276. Online ahead of print.

Authors

Shu-Ya Wang¹, Maryamgul Tohti², Jia-Qi Zhang², Jun Li², De-Qiang Li³

Affiliations

¹ Xinjiang Key Laboratory of Agricultural Chemistry and Biomaterials, College of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumchi 830052, Xinjiang, People's Republic of China; School of Bioengineering, Dalian University of Technology, Dalian 116024, Liaoning, People's Republic of China.
² Xinjiang Key Laboratory of Agricultural Chemistry and Biomaterials, College of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumchi 830052, Xinjiang, People's Republic of China.
³ Xinjiang Key Laboratory of Agricultural Chemistry and Biomaterials, College of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumchi 830052, Xinjiang, People's Republic of China. Electronic address: lsx20131120a@163.com.

PMID: 37582429
DOI: 10.1016/j.ijbiomac.2023.126276

Abstract

Injectable hydrogel-based drug delivery systems have attracted more and more attention due to their sustained-release performance, biocompatibility, and 3D network. The present study showed whole pectin-based hydrogel as an injectable drug delivery system, which was developed from oxidized pectin (OP) and diacylhydrazine adipate-functionalized pectin (Pec-ADH) via acylhydrazone linkage. The as-prepared hydrogels were characterized by ¹H NMR, FT-IR, and SEM techniques. The equilibrium swelling ratio of obtained hydrogel (i.e., sample gel 5) was up to 4306.65 % in the distilled water, which was higher than that in PBS with different pH values. Increasing the pH of the swelling media, the swelling ratio of all hydrogels decreased significantly. The results that involved the swelling properties indicated the salt- and pH-responsiveness of the as-prepared hydrogels. The drug release study presented that 5-FU can be persistently released for more than 12 h without sudden release. Moreover, the whole pectin-based hydrogel presented high cytocompatibility toward L929 cell lines, and the drug delivery system showed a high inhibitory effect on MCF-7 cell lines. All these results manifested that the acylhydrazone-derived whole pectin-based hydrogel was an excellent candidate for injectable drug delivery systems.

Keywords: Acylhydrazone linkage; Drug delivery system; Injectable hydrogel; Pectin; Self-healing.