The association between CD46 expression in B cells and the pathogenesis of airway allergy

Xiaorui Geng; Jinmei Xue; Haoyue Zheng; Limin Suo; Haotao Zeng; Miao Zhao; Shuo Song; Yu Liu; Changqing Zhao; Pingchang Yang

doi:10.1016/j.bbadis.2023.166845

The association between CD46 expression in B cells and the pathogenesis of airway allergy

Biochim Biophys Acta Mol Basis Dis. 2024 Jan;1870(1):166845. doi: 10.1016/j.bbadis.2023.166845. Epub 2023 Aug 12.

Authors

Xiaorui Geng¹, Jinmei Xue², Haoyue Zheng³, Limin Suo², Haotao Zeng¹, Miao Zhao¹, Shuo Song⁴, Yu Liu⁵, Changqing Zhao⁶, Pingchang Yang⁷

Affiliations

¹ Department of Otolaryngology, Longgang ENT Hospital and Shenzhen ENT Institute, Shenzhen, China.
² Department of Otolaryngology, Head & Neck Surgery, Second Hospital, Shanxi Medical University, Taiyuan, China.
³ Shenzhen Clinical School of Medicine, Guangzhou University of Chinese Medicine, Shenzhen, China; Institute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Allergy Division at Shenzhen University, Shenzhen, China.
⁴ Institute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Allergy Division at Shenzhen University, Shenzhen, China; Department of General Practical Medicine, Third Affiliated Hospital, Shenzhen University, Shenzhen, China.
⁵ Institute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Allergy Division at Shenzhen University, Shenzhen, China. Electronic address: 99776271@qq.com.
⁶ Department of Otolaryngology, Head & Neck Surgery, Second Hospital, Shanxi Medical University, Taiyuan, China. Electronic address: fahyj@126.com.
⁷ Shenzhen Clinical School of Medicine, Guangzhou University of Chinese Medicine, Shenzhen, China. Electronic address: pcy2356@szu.edu.cn.

PMID: 37579982
DOI: 10.1016/j.bbadis.2023.166845

Abstract

CD46 can facilitate the production of IgE. Activation of CD46 may contribute to the pathogenesis of allergic diseases. The aim of this study is to elucidate the association between CD46 expression in B cells and the pathogenesis of airway allergy. In this study, peripheral B cells were collected from a group of patients suffering from allergic rhinitis (AR). An AR mouse model was established to test the role of CD46 in the development of airway allergy. The results showed elevated amounts of IGE in peripheral CD46⁺ B cells of AR patients. CD46⁺ B cells of AR patients showed high reticulum endoplasmic (ER) stress status. The expression of CD46 in peripheral B cells was positively associated with the AR response in patients. The production of IgE in mice with airway allergy was prevented by ablating CD46 expression in B cells. Exposure to aluminum hydroxide up regulated the expression of Cd46 in B cells through exacerbating ER stress. Administration of Cd46 shRNA carrying nanoparticles attenuated experimental airway allergy. In conclusion, peripheral B cells in AR patients display elevated CD46 expression. Cd46 ablation in B cells can mitigate the production of IgE in mice and attenuate experimental airway allergy.

Keywords: Airway allergy; B cell; IgE; Immunity; Rhinitis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Humans
Immunoglobulin E*
Membrane Cofactor Protein / genetics
Mice
Rhinitis, Allergic* / metabolism

Substances

CD46 protein, human
Immunoglobulin E
Membrane Cofactor Protein
Mcp protein, mouse