Bipolar hemicyanine cationic probe for simultaneous sensing of ATP and GTP

Mengxing Niu; Xiaofei Liang; Haotian Zhao; Huixin Li; Xuancheng Fu; Chang Liu

doi:10.1016/j.saa.2023.123249

Bipolar hemicyanine cationic probe for simultaneous sensing of ATP and GTP

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15:303:123249. doi: 10.1016/j.saa.2023.123249. Epub 2023 Aug 9.

Authors

Mengxing Niu¹, Xiaofei Liang², Haotian Zhao³, Huixin Li⁴, Xuancheng Fu⁵, Chang Liu⁶

Affiliations

¹ School of Applied Engineering, Henan University of Science and Technology, Sanmenxia 472000, PR China.
² School of Music, Henan University, Kaifeng 475001, PR China.
³ Department of Physical Education, Jiangnan University, Wuxi 214122, PR China.
⁴ School of Sport Science, Beijing Sport University, Beijing 100084, PR China.
⁵ Department of Chemistry, Syracuse University, Syracuse 13244, United States. Electronic address: fuxuancheng@iccas.ac.cn.
⁶ School of Sport Science, Beijing Sport University, Beijing 100084, PR China. Electronic address: c.liu@bsu.edu.cn.

PMID: 37579665
DOI: 10.1016/j.saa.2023.123249

Abstract

Adenosine 5'-triphosphate (ATP) and guanosine 5'-triphosphate (GTP) are the most essential energy source in enormous biological processes. Various probes for ATP or GTP sensing, have been widely established, but the probe that could simultaneously monitor ATP and GTP is still rarely reported. Herein, we report a bipolar hemicyanine cationic probe for simultaneous sensing of ATP and GTP via a one-step monitoring process. This probe exhibited strong affinity to ATP and GTP through intramolecular electrostatic and π-π stacking interactions, which the binding constant on each step were determined as 6.15 × 10⁷ M^-1 and 1.57 × 10⁶ M^-1 for ATP, 3.19 × 10⁷ M^-1 and 3.81 × 10⁶ M^-1 for GTP. The sensitivity and specificity of this probe toward ATP or GTP over other twelve biological analogues (adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate (AMP), guanosine 5'-diphosphate (GDP), guanosine 5'-monophosphate (GMP), Etc.) have also been successfully demonstrated. Furthermore, due to the rapid response rate (within 10 s), we also proved that this probe could be employed as a monitor tool during the ATP or GTP-related enzymatic reaction process.

Keywords: ATP; Bipolar hemicyanine cationic probe; GTP; Simultaneous sensing; Ultra-rapid monitoring.

MeSH terms

Adenosine Triphosphate* / metabolism
Adenosine*
Guanosine Diphosphate / metabolism
Guanosine Triphosphate / metabolism

Substances

Guanosine Triphosphate
tetramethylene hemicyanine
triphosphoric acid
Adenosine Triphosphate
Guanosine Diphosphate
Adenosine