SMALLER TRICHOMES WITH VARIABLE BRANCHES (SVB) and its homolog SVBL act downstream of transcription factor NAC089 and function redundantly in Arabidopsis unfolded protein response

Abstract

The unfolded protein response (UPR) is a cellular mechanism that alleviates endoplasmic reticulum stress to maintain protein homeostasis. Although SMALLER TRICHOMES WITH VARIABLE BRANCHES (SVB) is characterized as an emerging UPR factor downstream of the IRE-bZIP60 pathway, whether its homologs participate in the plant UPR remains unknown. Here, we showed that an SVB homolog, SVB-like (SVBL), functions redundantly with SVB in endoplasmic reticulum stress tolerance. The svb-1 svbl-1 double mutant showed a hypersensitivity phenotype and had higher UPR gene expression under endoplasmic reticulum stress than single mutants and the wild type. SVB responded to endoplasmic reticulum stress by accumulating in the root epidermis and phloem cells, but SVBL did not. Ectopic expression of the UPR factor NAC089 up-regulated both SVB and SVBL genes, suggesting that SVB and SVBL work downstream of NAC089. Thus, SVB and SVBL play distinct roles that are modulated by the common upstream regulator NAC089 to cope with endoplasmic reticulum stress in Arabidopsis.

Keywords: Arabidopsis; SVB homologs; endoplasmic reticulum stress; phospholipid-binding protein; unfolded protein response.