A simple sequence repeats marker of disease resistance in shrimp Litopenaeus vannamei and its application in selective breeding

Bin Yin; Haiyang Wang; Shaoping Weng; Sedong Li; Jianguo He; Chaozheng Li

doi:10.3389/fgene.2023.1144361

A simple sequence repeats marker of disease resistance in shrimp Litopenaeus vannamei and its application in selective breeding

Front Genet. 2023 Jul 27:14:1144361. doi: 10.3389/fgene.2023.1144361. eCollection 2023.

Authors

Bin Yin^{1

2

3}, Haiyang Wang^{1

2

3}, Shaoping Weng^{1

2

3}, Sedong Li⁴, Jianguo He^{1

2

3

5}, Chaozheng Li^{1

2

3

5}

Affiliations

¹ State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Marine Sciences, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
² Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Guangzhou, China.
³ China-ASEAN Belt and Road Joint Laboratory on Marine Aquaculture Technology, Guangzhou, China.
⁴ Guangdong Evergreen Feed Industry Co., Ltd., Zhanjiang, China.
⁵ Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, China.

Abstract

The polymorphism of the simple sequence repeat (SSR) in the 5' untranslated coding region (5'-UTR) of the antiviral gene IRF (LvIRF) has been shown to be implicated in the resistance to viral pathogens in shrimp Litopenaeus vannamei (L. vannamei). In this study, we explored the potential of this (CT)n-SSR marker in disease resistance breeding and the hereditary property of disease resistance traits in offspring. From 2018 to 2021, eight populations were generated through crossbreeding by selecting individuals according to microsatellite genotyping. Our results demonstrated that shrimp with the shorter (CT)n repeat exhibited higher resistance to white spot syndrome virus (WSSV) or Decapod iridescent virus 1 (DIV1); meanwhile, these resistance traits could be inherited in offspring. Interestingly, we observed that the longer (CT)n repeats were associated with bacterial resistance traits. Accordingly, shrimp with longer (CT)n repeats exhibited higher tolerance to Vibrio parahaemolyticus infection. Taken together, these results indicate that the single (CT)n-SSR marker could be used to selective breeding for both resistance to virus and bacteria in shrimps.

Keywords: IRF; disease resistance; selective breeding; shrimp; simple sequence repeat (SSR).

Grants and funding

This research was supported by National Key Research and Development Program of China (2022YFD2400204), National Natural Science Foundation of China (32102777/32022085/31930113), the open competition program of top ten critical priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province (2022SDZG01), Independent Research and Development Projects of Maoming Laboratory (2021TDQD004), Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (SML2021SP301), and the Fundamental Research Funds for the Central Universities, Sun Yat-sen University (22lglj05).