Alginate coated biogenic silver nanoparticles for the treatment of Pseudomonas infections in rainbow trout

Viktoryia Kulikouskaya; Viktoryia Nikalaichuk; Kseniya Hileuskaya; Alena Ladutska; Karine Grigoryan; Irina Kozerozhets; Varduhi Hovsepyan; Mariam Sargsyan; Anastasiya Sidarenka

doi:10.1016/j.ijbiomac.2023.126302

Alginate coated biogenic silver nanoparticles for the treatment of Pseudomonas infections in rainbow trout

Int J Biol Macromol. 2023 Aug 12:251:126302. doi: 10.1016/j.ijbiomac.2023.126302. Online ahead of print.

Authors

Viktoryia Kulikouskaya¹, Viktoryia Nikalaichuk², Kseniya Hileuskaya², Alena Ladutska³, Karine Grigoryan⁴, Irina Kozerozhets⁵, Varduhi Hovsepyan⁶, Mariam Sargsyan⁵, Anastasiya Sidarenka³

Affiliations

¹ Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryna Str., 220084 Minsk, Belarus. Electronic address: kulikouskaya@gmail.com.
² Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 F. Skaryna Str., 220084 Minsk, Belarus.
³ Institute of Microbiology, National Academy of Sciences of Belarus, Minsk, Belarus, 2 Kuprevich Str., 220084 Minsk, Belarus.
⁴ Yerevan State University, 1 Alek Manukyan St, Yerevan 0025, Armenia.
⁵ Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, 31 Leninsky Prospect, 119991 Moscow, Russia.
⁶ Vanadzor State University, Tigran Mets 36, 2021 Vanadzor, Armenia.

PMID: 37573909
DOI: 10.1016/j.ijbiomac.2023.126302

Abstract

Pseudomonas species are among the main pathogens causing rainbow trout infections. The present study provides a simple, green, sustainable, and rapid technique to synthesize of biogenic alginate-capped silver nanoparticles (Alg-Ag NPs) suitable for the treatment of Pseudomonas infections. It has been shown that the mechanism (aggregative or autocatalytic) of Alg-Ag NPs formation depended on Alg concentration and the heating approach used. The rate constants and activation energy were calculated. Alg-Ag NPs were characterized by UV-Vis, FTIR, XRD, TEM, AFM, XPS, and DLS. The optimal conditions for the fabrication of spherically-shaped (17-19 nm) and negatively-charged (zeta-potential <-50 mV) Alg-Ag NPs, which are stable during 9 months, included hot-plate assisted synthesis at 100 °C in diluted (1 mg/mL) Alg solutions. In vitro studies showed that Alg-Ag NPs exhibited prominent antimicrobial activity against collection Pseudomonas strains (inhibition zones ranged from 9.0 ± 1.0 to 19.0 ± 1.0 mm), with no significant loss of antibacterial efficacy after 9 months of storage. AFM analysis confirmed that the antibacterial effect of Alg-Ag NPs dealt with the direct nanomechanical disrupting of bacterial cells. The ability of Alg-Ag NPs to inhibit the growth of virulent P.aeruginosa, P.fluorescens and P. putida strains isolated from infected rainbow trout was evaluated. All tested strains were susceptible to Alg(10)-Ag NPs, while Alg(1)-Ag NPs demonstrated a limited strain-specific antibacterial effect. The obtained data displayed the prospects for the application of biogenic Alg-Ag NPs to create novel delivery systems for combating Pseudomonas infections in rainbow trout.

Keywords: Alginate; Antibacterial activity; Pseudomonas; Rainbow trout; Silver nanoparticles; ‘Green’ chemistry.