Abstract
Ribosome biogenesis is a fundamental process in eukaryotic cells. NOTCHLESS (NLE) is involved in 60S ribosome biogenesis in yeast, but its role in Arabidopsis (A. thaliana) remains exclusive. Here, we found that Arabidopsis NLE (AtNLE) is highly conservative in phylogeny, which encoding a WD40-repeat protein. AtNLE is expressed in actively dividing tissues. AtNLE-GFP is localized in the nucleus. AtNLE physically interacts with the MIDAS domain of AtMDN1, a protein involved in the biogenesis of the 60S ribosomal subunit in Arabidopsis. The underexpressing mutant nle-2 shows short roots and reduced cell number in the root meristem. In addition, the null mutant nle-1 is embryo lethal, and defective embryos are arrested at the early globular stage. This work suggests that AtNLE interacts with AtMDN1, and AtNLE functions in root and embryo development.
Keywords:
Arabidopsis; MDN1; NLE; embryo; root.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis Proteins* / genetics
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Arabidopsis Proteins* / metabolism
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Arabidopsis* / metabolism
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Cell Nucleus / metabolism
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Embryonic Development
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Meristem / genetics
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Meristem / metabolism
Substances
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Arabidopsis Proteins
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AT5G52820 protein, Arabidopsis
Grants and funding
This work was supported by the National Natural Science Foundation of China (32100266), Shandong Natural Science Foundation (ZR2021QC022, ZR2020QC145 and ZR2022QC076), Shandong Academy of Agricultural Sciences, Introduction and Training of High-level Talents (CXGC2022E15, CXGC2022F15 and CXGC2023F15), Improved Variety Program of Shandong Province (2020LZGCQY019 and 2020LZGC008), the Key Research and Development Project of Shandong Province (2021LZGC025 and 2022CXGC010605), Scientific Research Guide Foundation of Shandong Academy of Grape (SDAG2021A01 and SDAG2021A02), Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2022F38, CXGC2023A22, CXGC2023A41, CXGC2023C02 and CXGC2023G32) and Yantai Science and Technology Plan Project(2022XCZX086).