Expression and immunogenicity of recombinant porcine epidemic diarrhea virus Nsp9

Shiguo Liu; Qijia Yu; Sha Li; Mingzhi Li; Li Yang; Quansheng Wang; Zewen Tu; Feifei Tao; Pingping Yang; Lingbao Kong; Xiu Xin

doi:10.1016/j.virol.2023.109861

Expression and immunogenicity of recombinant porcine epidemic diarrhea virus Nsp9

Virology. 2023 Oct:587:109861. doi: 10.1016/j.virol.2023.109861. Epub 2023 Aug 3.

Authors

Shiguo Liu¹, Qijia Yu¹, Sha Li¹, Mingzhi Li¹, Li Yang¹, Quansheng Wang¹, Zewen Tu¹, Feifei Tao¹, Pingping Yang¹, Lingbao Kong¹, Xiu Xin²

Affiliations

¹ Institute of Pathogenic Microorganism and College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China; Nanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang, 330045, China.
² Institute of Pathogenic Microorganism and College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China; Nanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang, 330045, China. Electronic address: xinxiujiangxi@sina.com.

PMID: 37572518
DOI: 10.1016/j.virol.2023.109861

Abstract

Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea, vomiting, dehydration, and high mortality in newborn piglets, which leads to significant economic losses. Coronavirus nonstructural protein 9 (Nsp9) is an essential RNA binding protein for coronavirus replication, which renders it a promising candidate for developing antiviral drugs and diagnosis targeting PEDV. In this study, PEDV Nsp9 protein fused with MBP protein and His-tag were expressed and purified in Escherichia coli. Furthermore, immunization of MBP-Nsp9 enhances both humoral and cellular immunity responses as compared with that of His-Nsp9 protein. Finally, the swine immunization showed that Nsp9 protein could stimulate the swine immunity system to carry out humoral immunity, and the generated antibody could inhibit the proliferation of PEDV in Vero cells. Altogether, our data provide direct evidence for the immunogenicity of PEDV Nsp9, which sheds light on the future developments of anti-PEDV drugs and vaccines for PED prevention.

Keywords: Immunogenicity; MBP; Nsp9; PEDV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chlorocebus aethiops
Coronavirus Infections* / prevention & control
Coronavirus Infections* / veterinary
Coronavirus*
Diarrhea
Immunization
Porcine epidemic diarrhea virus* / physiology
Swine
Swine Diseases*
Vaccination
Vero Cells