Characterization of PAMAM Dendrimers for the Delivery of Nucleic Acid Nanoparticles

Yelixza I Avila; Laura Rebolledo; Melanie Andrade-Muñoz; Kirill A Afonin

doi:10.1007/978-1-0716-3417-2_17

Characterization of PAMAM Dendrimers for the Delivery of Nucleic Acid Nanoparticles

Methods Mol Biol. 2023:2709:253-259. doi: 10.1007/978-1-0716-3417-2_17.

Authors

Yelixza I Avila¹, Laura Rebolledo¹, Melanie Andrade-Muñoz¹, Kirill A Afonin²

Affiliations

¹ Nanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC, USA.
² Nanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC, USA. kafonin@uncc.edu.

Abstract

The protocols described in this book chapter are meant to be used as an outline and guideline to explore the use of a cationic, polymeric, and synthetic carrier-poly (amidoamine) (PAMAM) dendrimers. The amine-terminated, hyperbranched structures have been identified as a vehicle for the delivery of nucleic acids. As such, clear protocols for the optimization of dendrimer usage should be set in place. This chapter details the experiments used to determine the ratio that dendrimers and nucleic acids should be complexed at through the use of binding assays, nuclease protection assays, and competitive binding assays.

Keywords: Binding assay; Carrier; Delivery; Gel electrophoresis; NANPs; Nuclease protection; PAMAM dendrimers.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Dendrimers* / chemistry
Nanoparticles* / chemistry
Nucleic Acids*
Polymers

Substances

PAMAM Starburst
Dendrimers
Nucleic Acids
Polymers

Grants and funding

R35 GM139587/GM/NIGMS NIH HHS/United States