Hyaluronic acid-mediated collagen intrafibrillar mineralization and enhancement of dentin remineralization

Haiyan Wu; Changyu Shao; Jue Shi; Zihe Hu; Yanyan Zhou; Zhuo Chen; Ruikang Tang; Zhijian Xie; Wenjing Jin

doi:10.1016/j.carbpol.2023.121174

Hyaluronic acid-mediated collagen intrafibrillar mineralization and enhancement of dentin remineralization

Carbohydr Polym. 2023 Nov 1:319:121174. doi: 10.1016/j.carbpol.2023.121174. Epub 2023 Jul 3.

Authors

Haiyan Wu¹, Changyu Shao¹, Jue Shi¹, Zihe Hu¹, Yanyan Zhou¹, Zhuo Chen¹, Ruikang Tang², Zhijian Xie³, Wenjing Jin⁴

Affiliations

¹ Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou 310000, China.
² Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
³ Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou 310000, China. Electronic address: xzj66@zju.edu.cn.
⁴ Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou 310000, China. Electronic address: wjjin@zju.edu.cn.

PMID: 37567692
DOI: 10.1016/j.carbpol.2023.121174

Abstract

Non-collagenous proteins (NCPs) in the extracellular matrix (ECM) of bone and dentin are known to play a critical regulatory role in the induction of collagen fibril mineralization and are embedded in hyaluronic acid (HA), which acts as a water-retaining glycosaminoglycan and provides necessary biochemical and biomechanical cues. Our previous study demonstrated that HA could regulate the mineralization degree and mechanical properties of collagen fibrils, yet its kinetics dynamic mechanism on mineralization is under debate. Here, we further investigated the role of HA on collagen fibril mineralization and the possible mechanism. The HA modification can significantly promote intrafibrillar collagen mineralization by reducing the electronegativity of the collagen surface to enhance calcium ions (Ca²⁺) binding capacity to create a local higher supersaturation. In addition, the HA also provides additional nucleation sites and shortens the induction time of amorphous calcium phosphate (ACP)-mediated hydroxyapatite (HAP) crystallization, which benefits mineralization. The acceleration effect of HA on intrafibrillar collagen mineralization is also confirmed in collagen hydrogel and in vitro dentin remineralization. These findings offer a physicochemical view of the regulation effect of carbohydrate polymers in the body on biomineralization, the fine prospect for an ideal biomaterial to repair collagen-mineralized tissues.

Keywords: Collagen fibrils; Dentin remineralization; Hyaluronic acid; Intrafibrillar mineralization.