Enhanced exopolysaccharide production of Cordyceps militaris via mycelial cell immobilization on plastic composite support in repeated-batch fermentation

Shin-Ping Lin; Ting-Hsuan Sung; Artik Elisa Angkawijaya; Alchris Woo Go; Chang-Wei Hsieh; Hsien-Yi Hsu; Shella Permatasari Santoso; Kuan-Chen Cheng

doi:10.1016/j.ijbiomac.2023.126267

Enhanced exopolysaccharide production of Cordyceps militaris via mycelial cell immobilization on plastic composite support in repeated-batch fermentation

Int J Biol Macromol. 2023 Oct 1:250:126267. doi: 10.1016/j.ijbiomac.2023.126267. Epub 2023 Aug 10.

Authors

Shin-Ping Lin¹, Ting-Hsuan Sung², Artik Elisa Angkawijaya³, Alchris Woo Go⁴, Chang-Wei Hsieh⁵, Hsien-Yi Hsu⁶, Shella Permatasari Santoso⁷, Kuan-Chen Cheng⁸

Affiliations

¹ School of Food Safety, Taipei Medical University, #250, Wuxing Street, Xinyi Dist., Taipei 11042, Taiwan; Research Center of Biomedical Device, Taipei Medical University, #250 Wu-Hsing Street, Taipei 11031, Taiwan; TMU Research Center for Digestive Medicine, Taipei Medical University, #250 Wu-Hsing Street, Taipei 11031, Taiwan; Ph.D. Program in Drug Discovery and Development Industry, Taipei Medical University, #250 Wu-Hsing Street, Taipei 11031, Taiwan; Taiwan Institute of Food Science and Technology, National Taiwan University, #1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan.
² Institute of Food Science and Technology, National Taiwan University, #1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan; School of Nutrition and Health Sciences, Taipei Medical University, #250 Wu-Hsing Street, Taipei 11031, Taiwan.
³ Center for Sustainable Resource Science, RIKEN, Yokohama 230-0045, Japan.
⁴ Department of Chemical Engineering, National Taiwan University of Science and Technology, #43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan.
⁵ Department of Food Science and Biotechnology, National Chung Hsing University, South Dist., Taichung City 40227, Taiwan; Department of Medical Research, China Medical University Hospital, North Dist., Taichung City 404333, Taiwan; Taiwan Institute of Biotechnology, National Taiwan University, #1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan.
⁶ School of Energy and Environment, Department of Materials Science and Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong, China; Shenzhen Research Institute of City University of Hong Kong, Shenzhen 518057, China.
⁷ Department of Chemical Engineering, Widya Mandala Surabaya Catholic University, Kalijudan 37, Surabaya 60114, Indonesia; Collaborative Research Center for Zero Waste and Sustainability, Jl. Kalijudan 37, Surabaya 60114, East Java, Indonesia. Electronic address: shella@ukwms.ac.id.
⁸ Institute of Food Science and Technology, National Taiwan University, #1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan; Institute of Biotechnology, National Taiwan University, #1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, 91, Hsueh-Shih Road, Taichung 40402, Taiwan; Department of Optometry, Asia University, 500, Lioufeng Rd., Wufeng, Taichung 41354, Taiwan. Electronic address: kccheng@ntu.edu.tw.

PMID: 37567526
DOI: 10.1016/j.ijbiomac.2023.126267

Abstract

Repeated-batch fermentation with fungal mycelia immobilized in plastic composite support (PCS) eliminates the lag phase during fermentation and improves metabolite productivity. The strategy is implemented herein, and a novel modified PCS is developed to enhance exopolysaccharide (EPS) production from the medicinal fungus Cordyceps militaris. A modified PCS (SYE + PCS) was made by compositing polypropylene (PP) with a nutrient mixture containing soybean hull, peptone, yeast extract, and minerals (SYE+). The use of SYE + PCS has consistent cell productivity throughout the multiple fermentation cycles, which resulted in a more higher cell productivity after second batch compared to unmodified PCS. The cell grown on SYE + PCS also generates a higher yield of EPS (3.36, 6.93, and 5.72 g/L in the first, second, and third fermentation cycles, respectively) up to three-fold higher than the cell immobilized on unmodified PCS. It is also worth noting that the EPS from mycelium grown on SYE + PCS contains up to 2.3-fold higher cordycepin than those on unmodified PCS. The presence of nutrients in SYE + PCS also affects the hydrophobicity and surface roughness of the PC, improving mycelial cell adhesion. This study also provides a preliminary antioxidant activity assessment of EPS from immobilized C. militaris grown with SYE + PCS.

Keywords: Cordycepin; Fermentation; Mycelium cell; Repeated batch; Submerged culture.