Molecular breeding of livestock for disease resistance

Fei Gao; Pan Li; Ye Yin; Xuguang Du; Gengsheng Cao; Sen Wu; Yaofeng Zhao

doi:10.1016/j.virol.2023.109862

Molecular breeding of livestock for disease resistance

Virology. 2023 Oct:587:109862. doi: 10.1016/j.virol.2023.109862. Epub 2023 Aug 4.

Authors

Fei Gao¹, Pan Li², Ye Yin³, Xuguang Du¹, Gengsheng Cao⁴, Sen Wu⁵, Yaofeng Zhao⁶

Affiliations

¹ State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, National Engineering Laboratory for Animal Breeding, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, China; Sanya Institute of China Agricultural University, Sanya, 572025, China.
² State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, National Engineering Laboratory for Animal Breeding, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, China; College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
³ State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, National Engineering Laboratory for Animal Breeding, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, China.
⁴ Henan Livestock Genome Editing and Biobreeding Engineering Research Center, School of Life Sciences, Henan University, Kaifeng, 475004, China.
⁵ State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, National Engineering Laboratory for Animal Breeding, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, China; Sanya Institute of China Agricultural University, Sanya, 572025, China. Electronic address: swu@cau.edu.cn.
⁶ State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, National Engineering Laboratory for Animal Breeding, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, China. Electronic address: yaofengzhao@cau.edu.cn.

PMID: 37562287
DOI: 10.1016/j.virol.2023.109862

Abstract

Animal infectious diseases pose a significant threat to the global agriculture and biomedicine industries, leading to significant economic losses and public health risks. The emergence and spread of viral infections such as African swine fever virus (ASFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and avian influenza virus (AIV) have highlighted the need for innovative approaches to develop resilient and disease-resistant animal populations. Gene editing technologies, such as CRISPR/Cas9, offer a promising avenue for generating animals with enhanced disease resistance. This review summarizes recent advances in molecular breeding strategies for generating disease-resistant animals, focusing on the development of disease-resistant livestock. We also highlight the potential applications of genome-wide CRISPR/Cas9 library screening and base editors in producing precise gene modified livestock for disease resistance in the future. Overall, gene editing technologies have the potential to revolutionize animal breeding and improve animal health and welfare.

Keywords: CRISPR/Cas9; Disease resistance; Livestock; Molecular breeding.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

African Swine Fever Virus*
Animals
CRISPR-Cas Systems
DNA Shuffling
Disease Resistance / genetics
Livestock
Porcine respiratory and reproductive syndrome virus*
Swine