Analysis of the complete plastomes of Albizia kalkora (Roxb.) Prain 1897 (Fabaceae)

Heyu Yang; Mei Jiang; Sihui Sun; Shengyu Liu; Haimei Chen; Qing Du; Bin Wang; Yong Li; Liqiang Wang; Chang Liu

doi:10.1080/23802359.2023.2241684

Analysis of the complete plastomes of Albizia kalkora (Roxb.) Prain 1897 (Fabaceae)

Mitochondrial DNA B Resour. 2023 Aug 7;8(8):841-846. doi: 10.1080/23802359.2023.2241684. eCollection 2023.

Authors

Heyu Yang¹, Mei Jiang¹, Sihui Sun², Shengyu Liu³, Haimei Chen¹, Qing Du^{1

4}, Bin Wang¹, Yong Li², Liqiang Wang⁵, Chang Liu¹

Affiliations

¹ Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from Ministry of Education, Engineering Research Center of Chinese Medicine Resources from Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, P. R. China.
² College of Pharmacy, Xiangnan University, Chenzhou, P. R. China.
³ Institute of Medical Information & Library, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
⁴ Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibetan Plateau in Qinghai Province, Qinghai Nationalities University, Xining, Qinghai, China.
⁵ College of Pharmacy, Heze University, Heze, Shandong Province, P.R. China.

Abstract

Albizia kalkora (Roxb.) Prain 1897, belonging to the family Fabaceae, is not only a landscape tree but also a medicinal plant. At present, few plastomes have been reported from Albizia, which delays the in-depth phylogenomic studies and the development of high-resolution discriminating markers for this genus. Herein, we sequenced the first plastome of A. kalkora by NGS technology. The genome is a circular structure (176,158 bp), containing a large single-copy (LSC) region (91,521 bp), a small copy (SSC) region (5237 bp), and two inverted repeat (IR) regions (39,700 bp each). It has 35.45% GC content and encodes 109 unique genes, which are 76 protein-coding, 4 rRNA, and 29 tRNA genes. The genetic distance analysis of the intergenic spacer regions for A. kalkora, A. odoratissima and A. bracteate shows four intergenic regions with very high K2p values, namely, ccsA-ndhD (15.04), matK-rps16 (10.77), rps11-rpl36 (17.63) and rps3-rps19 (20.08), which can discriminate the three Albizia species. In addition, we identified ten pairs of regions that could be utilized to design primers to discriminate the three Albizia species. The phylogenetic analysis showed Albizia was closely related to Samanea. The results in this study will provide valuable information to elucidate the classification, identification and evolutionary history of Albizia.

Keywords: Albizia kalkora; DNA barcode; fabaceae; phylogeny; plastome.

Grants and funding

This work was supported by funds from Chinese Academy of Medical Sciences, Innovation Funds for Medical Sciences (CIFMS) [2021-1-I2M-022], National Science Foundation [81872966], National Science &Technology Fundamental Resources Investigation Program of China [2018FY100705], Doctoral Fund Project of Heze University [XY20BS09], Shandong Provincial Natural Science Foundation [ZR2021MC136]. The funders were not involved in the study design, data collection and analysis, decision to publish, or manuscript preparation.