The identification of distinct retinal ganglion cell (RGC) populations in flat-mounted retinas is key to investigating pathological or pharmacological effects in these cells. In this chapter, we review the main techniques for detecting the total population of RGCs and various of their subtypes in whole-mounted retinas of pigmented and albino rats and mice, four of the animal strains most studied by the scientific community in the retina field. These methods are based on the studies published by the Vidal-Sanz's laboratory.
Keywords: Immunohistofluorescence; Mouse; Population; RGC markers; Rat; Retinal ganglion cells.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.