High-Throughput Binocular Pattern Electroretinograms in the Mouse

Tsung-Han Chou; Jonathon Toft-Nielsen; Vittorio Porciatti

doi:10.1007/978-1-0716-3409-7_16

High-Throughput Binocular Pattern Electroretinograms in the Mouse

Methods Mol Biol. 2023:2708:147-153. doi: 10.1007/978-1-0716-3409-7_16.

Authors

Tsung-Han Chou¹, Jonathon Toft-Nielsen², Vittorio Porciatti³

Affiliations

¹ Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
² JORVEC Corp, Miami, FL, USA.
³ Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA. vporciatti@med.miami.edu.

PMID: 37558969
DOI: 10.1007/978-1-0716-3409-7_16

Abstract

The pattern electroretinogram (PERG) reflects the electrical activity of retinal ganglion cells (RGC) and has become the most used technology to assess RGC function in experimental models of glaucoma and optic neuropathies. We describe a novel method for obtaining user-friendly, robust PERG simultaneously from each eye using asynchronous binocular stimulation and one-channel acquisition of signals recorded from a subcutaneous needle in the snout.

Keywords: Mouse; PERG; Pattern electroretinogram; Retinal ganglion cells.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Electroretinography* / methods
Glaucoma*
Mice
Photic Stimulation
Retinal Ganglion Cells / physiology

Grants and funding

R43 EY023460/EY/NEI NIH HHS/United States