Differentiation of Human Embryonic/Induced-Pluripotent Stem Cells to Retinal Ganglion Cells

Maryam Esmaeili; Ben Mead

doi:10.1007/978-1-0716-3409-7_5

Differentiation of Human Embryonic/Induced-Pluripotent Stem Cells to Retinal Ganglion Cells

Methods Mol Biol. 2023:2708:41-48. doi: 10.1007/978-1-0716-3409-7_5.

Authors

Maryam Esmaeili¹, Ben Mead²

Affiliations

¹ School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK. esmaeilim@cardiff.ac.uk.
² School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK.

PMID: 37558958
DOI: 10.1007/978-1-0716-3409-7_5

Abstract

The generation of retinal ganglion cells (RGCs) differentiated from human embryonic stem cell (hESC) or induced-pluripotent stem cells (iPSC) could aid with understanding of human RGC development, neuronal biology, drug discovery, potential cell-based therapies, and gene regulation. Here, we present a protocol for differentiation of hESC to RGCs using a 40-day protocol, significantly shorter than typical retinal organoids while still yielding cells with RGC-enriched markers and show physiological and morphological properties typical of RGCs.

Keywords: Human retinal ganglion cells; Pluripotent stem cells; Retinal organoid; Stem cells.

MeSH terms

Cell Differentiation
Cells, Cultured
Humans
Induced Pluripotent Stem Cells*
Retina
Retinal Ganglion Cells*