Ultra-high-performance liquid chromatography-tandem high-resolution elevated mass spectrometry profiling of anti-methicillin-resistant Staphylococcus aureus metabolites from the endophytic bacteria collected from the weeds of a previous dumpsite

Mona Lisa B Lacson; Carolyn A Arbotante; Ma Jamaica Trexy E Magdayao; Reynaldo Dl Bundalian Jr; Andrea Roxanne J Anas

doi:10.1016/j.chroma.2023.464228

Ultra-high-performance liquid chromatography-tandem high-resolution elevated mass spectrometry profiling of anti-methicillin-resistant Staphylococcus aureus metabolites from the endophytic bacteria collected from the weeds of a previous dumpsite

J Chromatogr A. 2023 Sep 13:1706:464228. doi: 10.1016/j.chroma.2023.464228. Epub 2023 Jul 20.

Authors

Mona Lisa B Lacson¹, Carolyn A Arbotante², Ma Jamaica Trexy E Magdayao³, Reynaldo Dl Bundalian Jr⁴, Andrea Roxanne J Anas⁵

Affiliations

¹ Center for Advanced Research and Innovation, Office of the Vice President for Research and Innovation, Angeles University Foundation, Pampanga 2009 Philippines; College of Allied Medical Professions, Angeles University Foundation, Pampanga 2009 Philippines. Electronic address: lacson.monalisa@auf.edu.ph.
² College of Arts and Sciences, Angeles University Foundation, Pampanga 2009 Philippines.
³ Applied Chemistry Laboratory Regional Research Center, University of the Philippines Visayas, Miag-ao, Iloilo 5023 Philippines.
⁴ Center for Advanced Research and Innovation, Office of the Vice President for Research and Innovation, Angeles University Foundation, Pampanga 2009 Philippines. Electronic address: bundalianrdjr.cas@auf.edu.ph.
⁵ Department of Brain Function, Division of Stress Adaptation and Protection, Research Institute of Environmental Medicine, Nagoya University, Chikusa-Ku, Nagoya 464-8601, Japan; Department of Molecular Pharmacokinetics, Division of Clinical Pharmacology, Graduate School of Medicine Nagoya University, Chikusa-Ku, Nagoya 464-8601, Japan. Electronic address: roxanne.jocsing.anas.andrea.s0@f.mail.nagoya-u.ac.jp.

PMID: 37556933
DOI: 10.1016/j.chroma.2023.464228

Abstract

The culturable endophytic bacteria from the weeds Cleome rutidosperma of the family Cleomaceae and Digitaria sanguinalis of the family Poaceae obtained from a previous dumpsite in Pampanga, Philippines have been assessed for their anti-methicillin-resistant Staphylococcus aureus (MRSA) activity, and the analytes with such activity should be identified. However, due to the limited amounts collected from the isolation process, 1.8 mg yield of compound 1 from the endophyte of C. rutidosperma and 1.2 mg of a mixture from the endophyte of D. sanguinalis were selected for LC-MS^E analysis. The production of compounds from the culturable endophytic bacteria Pseudomonas aeruginosa- determined by gene-sequencing, an untargeted and data-independent analysis (DIA) by ultra-high performance liquid chromatography-high resolution-elevated energy mass spectrometry (UHPLC-HR-MS^E) technique was employed to profile the metabolites present in the two high-performance liquid chromatography (HPLC) fractions. The analytes present from P. aeruginosa detected by UHPLC-HR-MS^E isolated from C. rutidosperma was phenazine-1-carboxylic acid (1), and for D. sanguinalis were chamigrenal (2), dialkyl resorcinol (3), and a pyoverdine elicitor (4). This study proves that UHPLC-HR-MS^E could identify the anti-MRSA constituents in P. aeruginosa from commensal weeds C. rutidosperma and D. sanguinalis. The UHPLC-HR-MS^E could help strengthen metabolomics antibacterial research and its related applications from a future perspective. Application of metabolomics research using UHPLC-HR-MS^E could enhance the rehabilitation of dumpsites by the microbial community present.

Keywords: Dumpsite; Endophyte; UHPLC-HR-MS(E); Ultra-high pressure liquid chromatography-tandem elevated mass spectrometry.

MeSH terms

Chromatography, High Pressure Liquid / methods
Chromatography, Liquid
Metabolomics / methods
Methicillin-Resistant Staphylococcus aureus*
Tandem Mass Spectrometry / methods