Complete genome sequence of the archetype bile acid 7α-dehydroxylating bacterium, Clostridium scindens VPI12708, isolated from human feces, circa 1980

Kelly Yovani Olivos Caicedo; Francelys V Fernandez-Materan; Alvaro G Hernandez; Steven L Daniel; Joao M P Alves; Jason M Ridlon

doi:10.1128/MRA.00029-23

Complete genome sequence of the archetype bile acid 7α-dehydroxylating bacterium, Clostridium scindens VPI12708, isolated from human feces, circa 1980

Microbiol Resour Announc. 2023 Sep 19;12(9):e0002923. doi: 10.1128/MRA.00029-23. Epub 2023 Aug 9.

Authors

Kelly Yovani Olivos Caicedo¹, Francelys V Fernandez-Materan^{2

3}, Alvaro G Hernandez⁴, Steven L Daniel⁵, Joao M P Alves¹, Jason M Ridlon^{2

3

6

7

8

9}

Affiliations

¹ Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo , São Paulo, Brazil.
² Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign , Urbana, Illinois, USA.
³ Department of Animal Sciences, University of Illinois Urbana-Champaign , Urbana, Illinois, USA.
⁴ Keck Center for Biotechnology, University of Illinois Urbana-Champaign , Urbana, Illinois, USA.
⁵ Department of Biological Sciences, Eastern Illinois University , Charleston, Illinois, USA.
⁶ Division of Nutritional Sciences, University of Illinois Urbana-Champaign , Urbana, Illinois, USA.
⁷ Cancer Center at Illinois , Urbana, Illinois, USA.
⁸ Center for Advanced Study, University of Illinois Urbana-Champaign , Urbana, Illinois, USA.
⁹ Department of Microbiology and Immunology, Virginia Commonwealth University , Richmond, Virginia, USA.

Abstract

Clostridium scindens strain VPI12708 serves as model organism to study bile acid 7α-dehydroxylating pathways. The closed circular genome of C. scindens VPI12708 was obtained by PacBio sequencing. The genome is composed of 3,983,052 bp, with 47.59% G + C, and 3,707 coding DNA sequences are predicted.

Keywords: 7α-dehydroxylating bacteria; Clostridium scindens; bile acid; genome assembly; genome sequencing.

Grants and funding

R03 AI147127/AI/NIAID NIH HHS/United States