Role of helicity in the nonenzymatic template-directed primer extension of DNA

Sung Joon Park; Kimberley Laura Callaghan; Amanda Vera Ellis

doi:10.1039/d3ob01179h

Role of helicity in the nonenzymatic template-directed primer extension of DNA

Org Biomol Chem. 2023 Aug 23;21(33):6702-6706. doi: 10.1039/d3ob01179h.

Authors

Sung Joon Park¹, Kimberley Laura Callaghan¹, Amanda Vera Ellis¹

Affiliation

¹ School of Chemical and Biomedical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. amanda.ellis@unimelb.edu.au.

PMID: 37555399
DOI: 10.1039/d3ob01179h

Abstract

Complexing a DNA primer with an RNA template showed improved nonenzymatic template-directed primer extension, attributed to a shift in the DNA helicity from a B-type towards an A-type helix. A 2-fold (deoxyadenosine) and 4.5-fold (deoxycytidine) increase in conversion from initial DNA primer to a primer + 1 nucleotide product was observed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA
DNA Primers
Nucleotides*
RNA* / genetics
Templates, Genetic

Substances

DNA Primers
Nucleotides
RNA
DNA