Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Oryza sativa L.) under cadmium stress

Lingwei Yang; Junchao Fang; Jingxin Wang; Suozhen Hui; Liang Zhou; Bo Xu; Yujuan Chen; Yuanyuan Zhang; Changkai Lai; Guiai Jiao; Zhonghua Sheng; Xiangjin Wei; Gaoneng Shao; Lihong Xie; Ling Wang; Ying Chen; Fengli Zhao; Shikai Hu; Peisong Hu; Shaoqing Tang

doi:10.3389/fpls.2023.1222288

Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Oryza sativa L.) under cadmium stress

Front Plant Sci. 2023 Jul 24:14:1222288. doi: 10.3389/fpls.2023.1222288. eCollection 2023.

Authors

Lingwei Yang¹, Junchao Fang¹, Jingxin Wang¹, Suozhen Hui¹, Liang Zhou¹, Bo Xu¹, Yujuan Chen¹, Yuanyuan Zhang¹, Changkai Lai¹, Guiai Jiao¹, Zhonghua Sheng¹, Xiangjin Wei¹, Gaoneng Shao¹, Lihong Xie¹, Ling Wang¹, Ying Chen¹, Fengli Zhao¹, Shikai Hu¹, Peisong Hu¹, Shaoqing Tang¹

Affiliation

¹ State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China.

Abstract

3-Ketoacyl-CoA synthase (KCS) is the key rate-limiting enzyme for the synthesis of very long-chain fatty acids (VLCFAs) in plants, which determines the carbon chain length of VLCFAs. However, a comprehensive study of KCSs in Oryza sativa has not been reported yet. In this study, we identified 22 OsKCS genes in rice, which are unevenly distributed on nine chromosomes. The OsKCS gene family is divided into six subclasses. Many cis-acting elements related to plant growth, light, hormone, and stress response were enriched in the promoters of OsKCS genes. Gene duplication played a crucial role in the expansion of the OsKCS gene family and underwent a strong purifying selection. Quantitative Real-time polymerase chain reaction (qRT-PCR) results revealed that most KCS genes are constitutively expressed. We also revealed that KCS genes responded differently to exogenous cadmium stress in japonica and indica background, and the KCS genes with higher expression in leaves and seeds may have functions under cadmium stress. This study provides a basis for further understanding the functions of KCS genes and the biosynthesis of VLCFA in rice.

Keywords: cadmium stress; gene family; rice; very long-chain fatty acids; β-ketoacyl-CoA synthase.

Grants and funding

This work was supported by the Zhejiang Provincial Science and Technology Project (LDQ23C130001, 2020R51007), the Key Research and Development Program of Zhejiang Province (2022C02011, 2021C02056-1), the National Natural Science Foundation of China (32188102, 32071991, and 31972961).