Complete genome sequences of two Bacillus thuringiensis serovar kurstaki strains isolated from Lebanon and Tunisia, highly toxic against lepidopteran larvae

Nancy Fayad; Rita Barssoum; Nathalie Marsaud; Rayan Nasseredine; Nouha Abdelmalek; Souad Rouis; Marie Ange Teste; Vincent Pailler; Veronique Gautier; Elodie Belmonte; César Arturo Aceves Lara; Julien Cescut; Luc Fillaudeau; Mireille Kallassy Awad

doi:10.1128/MRA.00060-23

Complete genome sequences of two Bacillus thuringiensis serovar kurstaki strains isolated from Lebanon and Tunisia, highly toxic against lepidopteran larvae

Microbiol Resour Announc. 2023 Sep 19;12(9):e0006023. doi: 10.1128/MRA.00060-23. Epub 2023 Aug 8.

Authors

Nancy Fayad^{1

2}, Rita Barssoum^{1

3}, Nathalie Marsaud^{3

4}, Rayan Nasseredine¹, Nouha Abdelmalek⁵, Souad Rouis⁶, Marie Ange Teste⁴, Vincent Pailler⁷, Veronique Gautier⁷, Elodie Belmonte⁷, César Arturo Aceves Lara³, Julien Cescut⁸, Luc Fillaudeau³, Mireille Kallassy Awad¹

Affiliations

¹ Laboratory of Biodiversity and Functional Genomics, Université Saint-Joseph de Beyrouth , Beirut, Lebanon.
² Multi-Omics Laboratory, School of Pharmacy, Lebanese American University , Byblos, Lebanon.
³ Toulouse Biotechnology Institute , Toulouse, France.
⁴ GenoToul GeT-BioPUCE , Toulouse, France.
⁵ Laboratoires Pharmaceutiques MédiS , Tunis, Tunisia.
⁶ Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax , Sfax, Tunisia.
⁷ Gentyane, GDEC, INRAE , Clermont-Ferrand, France.
⁸ Toulouse White Biotechnology , Toulouse, France.

Abstract

Bacillus thuringiensis-based products are key in the biopesticides market. Bacillus thuringiensis kurstaki strains Lip and BLB1 were isolated from Lebanese and Tunisian soils, respectively. These strains are highly toxic against lepidopteran larvae, Ephestia kuehniella. Here, we report Lip and BLB1 complete genomes, including their plasmid and toxin contents.

Keywords: Bacillus thuringiensis; PacBio; genomes; whole-genome sequencing.

Abstract

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