Advances in the detection of β-lactamase: A review

Qian Zhuang; Huijun Guo; Tian Peng; Enjie Ding; Hui Zhao; Qiulan Liu; Shiyin He; Guojie Zhao

doi:10.1016/j.ijbiomac.2023.126159

Advances in the detection of β-lactamase: A review

Int J Biol Macromol. 2023 Aug 6:251:126159. doi: 10.1016/j.ijbiomac.2023.126159. Online ahead of print.

Authors

Qian Zhuang¹, Huijun Guo², Tian Peng², Enjie Ding², Hui Zhao², Qiulan Liu², Shiyin He³, Guojie Zhao⁴

Affiliations

¹ Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning 110122, China; Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110122, China.
² General Party Branch of the Second Clinical Department, China Medical University, Shenyang, Liaoning 110122, China.
³ Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning 110122, China.
⁴ Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning 110122, China. Electronic address: gjzhao@cmu.edu.cn.

PMID: 37549760
DOI: 10.1016/j.ijbiomac.2023.126159

Abstract

β-lactamase, an enzyme secreted by bacteria, is the main resistant mechanism of Gram-negative bacteria to β-lactam antibiotics. The resistance of bacteria to β-lactam antibiotics can be evaluated by testing the activity of β-lactamase. Traditional phenotypic detection is a golden principle, but it is time-consuming. In recent years, many new methods have emerged, which improve the efficiency by virtue of their sensitivity, low cost, easy operation, and other advantages. In this paper, we systematically review these researches and emphasize their limits of detection, sample operation, and test duration. Noteworthily, some detection systems can identify the β-lactamase subtype conveniently. We mainly divide these tests into three categories to elaborate their characteristics and application status. Both advantages and disadvantages of these methods are discussed. Additionally, we analyze the recent 5 years published researches to predict the trend of development in this field.

Keywords: Detection methods; Drug resistance; β-Lactamase.

Publication types

Review