Pharmacological Basis for Antispasmodic, Bronchodilator, and Antidiarrheal Potential of Dryopteris ramosa (Hope) C. via In Vitro, In Vivo, and In Silico Studies

Iram Iqbal; Fatima Saqib; Muhammad Farhaj Latif; Hamna Shahzad; Lorena Dima; Bayan Sajer; Rosana Manea; Ciprian Pojala; Radu Necula

doi:10.1021/acsomega.3c01907

Pharmacological Basis for Antispasmodic, Bronchodilator, and Antidiarrheal Potential of Dryopteris ramosa (Hope) C. via In Vitro, In Vivo, and In Silico Studies

ACS Omega. 2023 Jul 20;8(30):26982-27001. doi: 10.1021/acsomega.3c01907. eCollection 2023 Aug 1.

Authors

Iram Iqbal¹, Fatima Saqib¹, Muhammad Farhaj Latif¹, Hamna Shahzad², Lorena Dima³, Bayan Sajer⁴, Rosana Manea³, Ciprian Pojala³, Radu Necula³

Affiliations

¹ Department of Pharmacology; Bahauddin Zakariya University, Multan 60000, Pakistan.
² Department of Biochemistry, Bahauddin Zakariya University, Multan 60000, Pakistan.
³ Faculty of Medicine, Transilvania University of Brasov, Brasov 500036, Romania.
⁴ Department of Biological Sciences, College of Science, King Abdulaziz University, Jeddah 80200, Saudi Arabia.

Abstract

Background:Dryopteris ramosa is used as an old treatment for several diseases. D. ramose fronds are eaten to treat gastrointestinal (GIT) issues and as an antibiotic. However, there is a dearth of literature justifying its traditional use. Aims and objectives: the current work used biological and molecular docking studies to support traditional usage and elucidate D. ramosa's multitarget mechanism. Materials and methods: Bioactive compounds were docked in silico. Force displacement transducers coupled with a power lab data gathering system examined the effects of compounds on rabbit jejunum, trachea, and aorta tissues. Albino mice and rats were used for in vivo studies. Results: Bioactive compounds interacted with inflammation, asthma, and diarrhea genes, according to in silico studies. D. ramosa crude extract (Dr.Cr) calmed impulsive contractions and K+ (80 mM)-provoked contractions in the jejunum and tracheal tissue dose-dependently, showing the presence of the Ca++ channel-blocking (CCB) effect, further verified by the rightward parallel shift of CRCs equivalent to verapamil. Polarity-based fractionation showed spasmolytic activity in Dr.DCM and muscarinic receptors mediated spasmogenic activity in the Dr.Aq fraction. Dr.Cr vasoconstricted the aortic preparation, which was totally blocked by an angiotensin II receptor antagonist. This suggests that Dr. Cr's contractile effect is mediated through angiotensin receptors. In rats and mice, it showed anti-inflammatory and antidiarrheal action. Conclusion: This study supports the traditional medicinal uses of D. ramosa against GIT disorders and may be an important therapeutic agent in the future.