P63 and Ki-67 expression in radicular cyst

Mohammed Amjed Alsaegh; Okba Mahmoud; Sudhir Rama Varma; Asok Mathew; Alaa Muayad Altaie; Shengrong Zhu

doi:10.1016/j.jobcr.2023.06.008

P63 and Ki-67 expression in radicular cyst

J Oral Biol Craniofac Res. 2023 Sep-Oct;13(5):575-580. doi: 10.1016/j.jobcr.2023.06.008. Epub 2023 Jul 29.

Authors

Mohammed Amjed Alsaegh¹, Okba Mahmoud^{2

3}, Sudhir Rama Varma^{2

3}, Asok Mathew^{2

3}, Alaa Muayad Altaie⁴, Shengrong Zhu⁵

Affiliations

¹ Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates.
² Department of Clinical Sciences, College of Dentistry, Ajman University, Ajman, United Arab Emirates.
³ Center for Medical and Bio-allied Health Sciences Research, Ajman University, Ajman, United Arab Emirates.
⁴ Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.
⁵ Department of Oral and Maxillofacial Surgery, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, PR China.

Abstract

Objectives: The aim of the current study was to identify the expression of P63 and its relation to odontogenic epithelial cell proliferation, severity of the inflammatory infiltrate and size of radicular cysts (RCs).

Methods: In this retrospective cross-sectional study, 30 cases of paraffin-embedded RCs were randomly selected from the archive. P63 and Ki-67 expression was assessed by immunohistochemistry.

Results: Epithelial P63 expression was absent in four (13.3%), weak in 10 (33.3%), and moderate in 16 (53.3%) cases. In the connective tissue wall of RC, P63 expression was absent in two (6.7%) cases, weak in 24 (80.0%) cases, and moderate in four (13.3%) cases. Ki-67 was found to be weakly expressed in 12 (40.0%) cases, moderately expressed in 13 (43.3%), and strongly expressed in five (16.7%) cases. No correlation was found between Ki-67 expression in odontogenic epithelium and P63 expression in the odontogenic epithelium (rho = 0.110, p = .563) or fibrous capsule (rho = 0.160, p = .399). Nevertheless, we found a positive correlation between Ki-67 expression in the odontogenic epithelium and the size of the RC (rho = 0.450, p = .013). The inflammatory infiltrate was negatively correlated with P63 expression in the odontogenic epithelium (rho = -0.428, p = .018), and with the size of cysts (rho = -0.728, p < .001).

Conclusions: There is a high expression of P63 throughout the odontogenic epithelium and connective tissue capsule of the RC. P63 expression in the odontogenic epithelium is negatively correlated with the degree of the inflammatory infiltrate but not with epithelial cell proliferation or the size of the cyst.

Keywords: Ki-67; Odontogenic; P63; Proliferation; Radicular cyst.