Selenomethionine against titanium particle-induced osteolysis by regulating the ROS-dependent NLRP3 inflammasome activation via the β-catenin signaling pathway

Ruixuan Yu; Yongjian Yuan; Zhicheng Liu; Long Liu; Zhaoning Xu; Yunpeng Zhao; Chunwang Jia; Pengfei Zhang; Hang Li; Yuhao Liu; Yi Wang; Weiwei Li; Lin Nie; Xuecheng Sun; Yuhua Li; Ben Liu; Haichun Liu

doi:10.3389/fimmu.2023.1171150

Selenomethionine against titanium particle-induced osteolysis by regulating the ROS-dependent NLRP3 inflammasome activation via the β-catenin signaling pathway

Front Immunol. 2023 Jul 20:14:1171150. doi: 10.3389/fimmu.2023.1171150. eCollection 2023.

Authors

Ruixuan Yu¹, Yongjian Yuan¹, Zhicheng Liu^{1

2}, Long Liu³, Zhaoning Xu⁴, Yunpeng Zhao¹, Chunwang Jia¹, Pengfei Zhang¹, Hang Li¹, Yuhao Liu¹, Yi Wang^{5

6}, Weiwei Li³, Lin Nie¹, Xuecheng Sun⁷, Yuhua Li¹, Ben Liu¹, Haichun Liu¹

Affiliations

¹ Department of Orthopaedics, Qilu Hospital of Shandong University, Jinan, Shandong, China.
² The First Clinical Medical School, Shandong University, Jinan, Shandong, China.
³ Department of Pathology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.
⁴ School of Nursing and Rehabilitation, Shandong University, Jinan, Shandong, China.
⁵ Department of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, Shandong, China.
⁶ Emergency Medicine Center, The Second Hospital of Shandong University, Jinan, Shandong, China.
⁷ Department of Orthopedic Trauma, Weifang People's Hospital, Weifang, Shandong, China.

Abstract

Wear debris-induced osteolysis, especially titanium (Ti) particles-induced osteolysis, is the most common cause of arthroplasty failure with no effective therapy. Previous studies have suggested that inflammation and impaired osteogenesis are associated with Ti particles -induced osteolysis. Selenium (Se) is an essential trace element in the human body, which forms selenomethionine (Se-Met) in nature, and selenoproteins has strong anti-inflammatory and antioxidant stress effects. In this study, the effects of Se-Met on Ti particles-induced osteolysis were observed and the potential mechanism was explored. We found that exogenous Se-Met relieved osteolysis induced by Ti particles in two animal models and MC3T3-E1 cells. We found that the addition of Se-Met effectively inhibited Ti particle-induced inflammation by regulating reactive oxygen species-dependent (ROS-dependent) NOD-like receptor protein 3 (NLRP3) inflammasome activation. These therapeutic effects were abrogated in MC3T3-E1 cells that had received a β-catenin antagonist, suggesting that Se-Met alleviates inflammatory osteolysis via the β-catenin signaling pathway. Collectively, these findings indicated that Se-Met may serve as a potential therapeutic agent for treating Ti particle-induced osteolysis.

Keywords: NLRP3; inflammatory osteolysis; selenomethionine; titanium particle-induced osteolysis; β-catenin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Animals
Inflammasomes
Inflammation / chemically induced
Mice
NLR Family, Pyrin Domain-Containing 3 Protein
Osteolysis* / chemically induced
Osteolysis* / metabolism
Reactive Oxygen Species
Selenomethionine* / metabolism
Signal Transduction
Titanium* / adverse effects
beta Catenin / antagonists & inhibitors
beta Catenin / metabolism

Substances

beta Catenin
Inflammasomes
NLR Family, Pyrin Domain-Containing 3 Protein
Reactive Oxygen Species
Selenomethionine
Titanium

Grants and funding

National Natural Science Foundation of China (grant No. 82072478 to Yunpeng Zhao, grant No. 82073437 to Weiwei Li), Shandong Provincial Natural Science Foundation (grant No. ZR2020YQ54 to Yunpeng Zhao).