Mining is one of the major contributors for land degradation and severe heavy metals based soil pollution. In this study, the physicochemical properties of magnesite mine soil was investigated and assess the optimistic and eco-friendly remediation approach with Hibiscus rosa-sinensis with the effect of pre-isolated Acidithiobacillus thiooxidans. The physicochemical properties analysis results revealed that most the parameter were either too less or beyond the permissible limits. The pre-isolated A. thiooxidans showed remarkable multi-metal tolerance up to 800 μg mL-1 concentration of Cr, Cd, Pb, and Mn. Heavy metal content in polluted soil was reduced to avoid more metal toxicity by diluting with fertile control soil as 80:20 and 60:40. The standard greenhouse experiment was performed to evaluate the phytoextraction potential of H. rosa-sinensis under the influence of A. thiooxidans in various treatment groups (G-I to G-V). The outcome of this investigation was declared that the multi-metal tolerant A. thiooxidans from G-III and G-II showed remarkable effect on growth and phytoextraction ability of H. rosa-sinensis on metal polluted magnesite mine soil in 180 d greenhouse study. These results suggested that the combination of H. rosa-sinensis and A. thiooxidans could be used as an excellent hyper-accumulator to extract metal pollution from polluted soil.
Keywords: A. thiooxidans; Hibiscus rosa-sinensis; Metal pollution; Metal tolerance; Phytoextraction.
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