Effect of intranasal oxytocin on palatable food consumption and c-Fos immunoreactivity in relevant brain areas in rats

Donisha S N K Liyanagamage; Laura K McColl; Lisa N M Glasgow; Allen S Levine; Pawel K Olszewski

doi:10.1016/j.physbeh.2023.114318

Effect of intranasal oxytocin on palatable food consumption and c-Fos immunoreactivity in relevant brain areas in rats

Physiol Behav. 2023 Nov 1:271:114318. doi: 10.1016/j.physbeh.2023.114318. Epub 2023 Aug 4.

Authors

Donisha S N K Liyanagamage¹, Laura K McColl¹, Lisa N M Glasgow¹, Allen S Levine², Pawel K Olszewski³

Affiliations

¹ Faculty of Science and Engineering, University of Waikato, Hamilton 3240, New Zealand.
² Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108, United States of America.
³ Faculty of Science and Engineering, University of Waikato, Hamilton 3240, New Zealand; Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108, United States of America; Department of Integrative Biology and Physiology, Medical School, University of Minnesota, Minneapolis, MN 55414, United States of America. Electronic address: pawel.olszewski@waikato.ac.nz.

PMID: 37543105
DOI: 10.1016/j.physbeh.2023.114318

Abstract

Peripheral and central injections of oxytocin (OT) in laboratory animals decrease eating for energy and palatability, but the hypophagic response is dependent on the administration route. Human studies rely on intranasal (IN) administration of the peptide, the route underutilized in OT animal feeding studies thus far. Therefore, we examined the effect of IN OT on various aspects of food consumption in rats: (a) overnight deprivation-induced standard chow intake, (b) episodic (2-h) consumption of calorie-dense and palatable high-fat high-sugar (HFHS) chow, (c) 2-h episodic intake of palatable and calorie-dilute sucrose and Intralipid solutions, and (d) 2-h sucrose solution intake in rats habituated to ingesting this solution daily for several weeks. Finally, we assessed c-Fos changes in response to the acute IN OT administration in rats habituated to daily sugar consumption. We found that IN 20μg OT decreased deprivation-induced intake of standard chow and HFHS chow in nondeprived rats without affecting water consumption. IN OT also reduced 2-hour episodic fluid consumption of sucrose, but not Intralipid. In the habitual sugar consumption paradigm, acute IN OT diminished sucrose solution intake in animals accustomed to the 2-hour/day sucrose meal regimen. In rats habitually consuming sucrose, IN OT altered c-Fos immunoreactivity in brain areas related to energy homeostasis and reward, including the central nucleus of the amygdala, the hypothalamic paraventricular and the arcuate nuclei. We conclude that IN OT is an effective appetite suppressant for carbohydrate/sugar diets in rats and its effects involve feeding-related brain circuits.

Keywords: Appetite; Food intake; Meal; Oxytocin; Rats.