Inhibitory effect of plain and functionalized graphene nanoplateles on hen egg white lysozyme fibrillation

Sakineh Abbaspour; Saeid Hadi Alijanvand; Dina Morshedi; Seyed Abbas Shojaosadati

doi:10.1016/j.colsurfb.2023.113487

Inhibitory effect of plain and functionalized graphene nanoplateles on hen egg white lysozyme fibrillation

Colloids Surf B Biointerfaces. 2023 Oct:230:113487. doi: 10.1016/j.colsurfb.2023.113487. Epub 2023 Aug 1.

Authors

Sakineh Abbaspour¹, Saeid Hadi Alijanvand², Dina Morshedi², Seyed Abbas Shojaosadati³

Affiliations

¹ Biotechnology Group Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box: 14115-111, Tehran, the Islamic Republic of Iran; Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965-161, Tehran, the Islamic Republic of Iran.
² Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965-161, Tehran, the Islamic Republic of Iran.
³ Biotechnology Group Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box: 14115-111, Tehran, the Islamic Republic of Iran. Electronic address: shoja_sa@modares.ac.ir.

PMID: 37542838
DOI: 10.1016/j.colsurfb.2023.113487

Abstract

Protein fibrillation is a phenomenon associated with misfolding and the production of highly ordered nanofibrils, which may cause serious degenerative diseases such as Parkinson's disease, Alzheimer's disease, and type 2 diabetes. Upon contact with biological fluids, the nanomaterials are immediately covered by proteins and interact with them. In this study, the effects of Graphene NanoPlateles (Plain-GNPs) and their modified forms with a carboxyl group (GNPs -COOH) and an amine group (GNPs -NH₂) are evaluated on the fibrillation process of Hen Egg White Lysozyme (HEWL). The fibrillation process of HEWL was studied using thioflavin-T, Circular Dichroism spectrometry, and Atomic Force Microscopy. Plain-GNPs significantly decreased the fibrillation process at different stages, including nucleation, exponential fibrillation phases, and end-mature fibril products. However, GNPs-COOH and GNPs-NH₂ affected the final fluorescence of ThT. The species formed in the presence of Plain-GNPs showed less toxicity in SH-SY5Y cells, which could be applicable for therapeutic purposes.

Keywords: Cytotoxicity; Graphene nano plateles; Lysozyme; Protein fibrillation; Surface chemistry.

MeSH terms

Amyloid / chemistry
Animals
Chickens / metabolism
Diabetes Mellitus, Type 2*
Egg White
Graphite* / chemistry
Graphite* / pharmacology
Muramidase / chemistry
Neuroblastoma*

Substances

Amyloid
Graphite
Muramidase