Fecal microbiota of the synanthropic golden jackal (Canis aureus)

Roi Lapid; Yair Motro; Hillary Craddock; Boris Khalfin; Roni King; Gila Kahila Bar-Gal; Jacob Moran-Gilad

doi:10.1186/s42523-023-00259-3

Fecal microbiota of the synanthropic golden jackal (Canis aureus)

Anim Microbiome. 2023 Aug 5;5(1):37. doi: 10.1186/s42523-023-00259-3.

Authors

Roi Lapid¹, Yair Motro², Hillary Craddock², Boris Khalfin², Roni King³, Gila Kahila Bar-Gal¹, Jacob Moran-Gilad⁴

Affiliations

¹ The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.B. 12, 7610001, Rehovot, Israel.
² Department of Health Policy and Management, School of Public Health, Faculty of Health Sciences, Ben-Gurion University of the Negev, 8410501, Beer-Sheva, Israel.
³ Science and Conservation Division, Israel Nature and Parks Authority, 3 Am Ve'Olamo St., 95463, Jerusalem, Israel.
⁴ Department of Health Policy and Management, School of Public Health, Faculty of Health Sciences, Ben-Gurion University of the Negev, 8410501, Beer-Sheva, Israel. giladko@post.bgu.ac.il.

Abstract

The golden jackal (Canis aureus), is a medium canid carnivore widespread throughout the Mediterranean region and expanding into Europe. This species thrives near human settlements and is implicated in zoonoses such as rabies. This study explores for the first time, the golden jackal fecal microbiota. We analyzed 111 fecal samples of wild golden jackals using 16S rRNA amplicon sequencing the connection of the microbiome to animal characteristics, burden of pathogens and geographic and climate characteristics. We further compared the fecal microbiota of the golden jackal to the black-backed jackal and domestic dog. We found that the golden jackal fecal microbiota is dominated by the phyla Bacteroidota, Fusobacteriota and Firmicutes. The golden jackal fecal microbiota was associated with different variables, including geographic region, age-class, exposure to rabies oral vaccine, fecal parasites and toxoplasmosis. A remarkable variation in the relative abundance of different taxa was also found associated with different variables, such as age-class. Linear discriminant analysis effect size (LEfSe) analysis found abundance of specific taxons in each region, Megasphaera genus in group 1, Megamonas genus in group 2 and Bacteroides coprocola species in group 3. We also found a different composition between the fecal microbiota of the golden jackal, blacked-backed jackal and the domestic dog. Furthermore, LEfSe analysis found abundance of Fusobacterium and Bacteroides genera in the golden jackal, Clostridia class in blacked-backed jackal and Megamonas genus in domestic dog. The golden jackal fecal microbiota is influenced by multiple factors including host traits and pathogen burden. The characterization of the microbiota of this thriving species may aid in mapping its spread and proximity to human settlements. Moreover, understanding the jackal microbiota could inform the study of potential animal and human health risks and inform control measures.

Keywords: 16S rRNA amplicon sequencing; Fecal microbiota; Golden jackal (Canis aureus); Israel; Microbiome; One-health; Wildlife; Zoonotic diseases.