Preparation of polysaccharide-based nanoparticles by chitosan and flaxseed gum polyelectrolyte complexation as carriers for bighead carp (Aristichthys nobilis) peptide delivery

Changliang Zheng; Mengting Chen; Yashu Chen; Yinghong Qu; Wenzheng Shi; Liu Shi; Yu Qiao; Xin Li; Xiaojia Guo; Lan Wang; Wenjin Wu

doi:10.1016/j.ijbiomac.2023.126121

Preparation of polysaccharide-based nanoparticles by chitosan and flaxseed gum polyelectrolyte complexation as carriers for bighead carp (Aristichthys nobilis) peptide delivery

Int J Biol Macromol. 2023 Sep 30:249:126121. doi: 10.1016/j.ijbiomac.2023.126121. Epub 2023 Aug 3.

Authors

Changliang Zheng¹, Mengting Chen², Yashu Chen³, Yinghong Qu⁴, Wenzheng Shi⁴, Liu Shi², Yu Qiao², Xin Li², Xiaojia Guo², Lan Wang⁵, Wenjin Wu⁶

Affiliations

¹ Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China.
² Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
³ Key Laboratory of Oilseeds Processing, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
⁴ College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China.
⁵ Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Electronic address: lilywang_2016@163.com.
⁶ Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Electronic address: wuwenjin@hbass.com.

PMID: 37541467
DOI: 10.1016/j.ijbiomac.2023.126121

Abstract

Polysaccharide-based nanoparticles formed by the polyelectrolyte complexation between chitosan (CS) and flaxseed gum (FG) was developed in this work, and it was further used as a carrier for bighead carp peptide (BCP) delivery. The CS molecular weight (M_W) of 50 kDa and CS/FG mass ratio of 1:2 at pH 3.5 were optimal conditions for the NP preparation, with the minimum particle size (∼155.1 nm) and the maximum BCP encapsulation efficiency (60.3 %). The BCP-loaded CS/FG NPs exhibited the smallest particle size (175.8 nm). Both CS/FG NPs and CS/FG-BCP NPs exhibited roughly uniform spherical shape. FT-IR spectra confirmed the existence of hydrogen bonds and electrostatic interactions in the nanoparticles. The BCP-loaded NPs displayed a higher thermal stability than BCP. Moreover, the release of BCP was controllable and dose-dependent, following a first-order kinetics model. These findings suggested that our CS/FG NPs are a promising carrier for bioactive peptide delivery.

Keywords: Bighead carp peptide; Chitosan; Control release; Flaxseed gum; Polyelectrolyte complexation.

MeSH terms

Animals
Carps*
Chitosan* / chemistry
Drug Carriers / chemistry
Flax*
Nanoparticles* / chemistry
Particle Size
Peptides / chemistry
Polyelectrolytes
Polysaccharides
Spectroscopy, Fourier Transform Infrared

Substances

Drug Carriers
Polyelectrolytes
Chitosan
Polysaccharides
Peptides