KCC2 reverse mode helps to clear postsynaptically released potassium at glutamatergic synapses

Abstract

Extracellular potassium [K⁺]_o elevation during synaptic activity retrogradely modifies presynaptic release and astrocytic uptake of glutamate. Hence, local K⁺ clearance and replenishment mechanisms are crucial regulators of glutamatergic transmission and plasticity. Based on recordings of astrocytic inward rectifier potassium current I_Kir and K⁺-sensitive electrodes as sensors of [K⁺]_o as well as on in silico modeling, we demonstrate that the neuronal K⁺-Cl^- co-transporter KCC2 clears local perisynaptic [K⁺]_o during synaptic excitation by operating in an activity-dependent reversed mode. In reverse mode, KCC2 replenishes K⁺ in dendritic spines and complements clearance of [K⁺]_o, therewith attenuating presynaptic glutamate release and shortening LTP. We thus demonstrate a physiological role of KCC2 in neuron-glial interactions and regulation of synaptic signaling and plasticity through the uptake of postsynaptically released K⁺.

Keywords: CP: Neuroscience; Slc12a5; cation-chloride cotransport; co-transport reversal; excitation balance; neuron-glial interactions; potassium uptake; synaptic potentiation.