Lactiplantibacillus plantarum, lactiplantibacillus pentosus and inulin meal inclusion boost the metagenomic function of broiler chickens

Ilario Ferrocino; Ilaria Biasato; Sihem Dabbou; Elena Colombino; Kalliopi Rantsiou; Simone Squara; Marta Gariglio; Maria Teresa Capucchio; Laura Gasco; Chiara Emilia Cordero; Erica Liberto; Achille Schiavone; Luca Cocolin

doi:10.1186/s42523-023-00257-5

Lactiplantibacillus plantarum, lactiplantibacillus pentosus and inulin meal inclusion boost the metagenomic function of broiler chickens

Anim Microbiome. 2023 Aug 3;5(1):36. doi: 10.1186/s42523-023-00257-5.

Affiliations

¹ Department of Agricultural, Forest and Food Sciences, University of Turin, Turin, Italy.
² Center Agriculture Food Environment (C3A), University of Trento, Turin, Italy.
³ Department of Veterinary Sciences, University of Turin, Turin, Italy.
⁴ Department of Drug Science and Technology, University of Turin, Turin, Italy.
⁵ Department of Veterinary Sciences, University of Turin, Turin, Italy. achille.schiavone@unito.it.
⁶ Department of Agricultural, Forest and Food Sciences, University of Turin, Turin, Italy. lucasimone.cocolin@unito.it.

Abstract

Background: The inclusion of alternative ingredients in poultry feed is foreseen to impact poultry gut microbiota. New feeding strategies (probiotics/prebiotics) must be adopted to allow sustainable productions. Therefore, the current study aimed to use metagenomics approaches to determine how dietary inclusion of prebiotic (inulin) plus a multi-strain probiotic mixture of Lactiplantibacillus plantarum and Lactiplantibacillus pentosus affected microbiota composition and functions of the gastro-intestinal tract of the broilers during production. Fecal samples were collected at the beginning of the trial and after 5, 11 and 32 days for metataxonomic analysis. At the end of the trial, broilers were submitted to anatomo-pathological investigations and caecal content was subjected to volatilome analysis and DNAseq.

Results: Probiotic plus prebiotic inclusion did not significantly influence bird performance and did not produce histopathological alterations or changes in blood measurements, which indicates that the probiotic did not impair the overall health status of the birds. The multi-strain probiotic plus inulin inclusion in broilers increased the abundance of Blautia, Faecalibacterium and Lachnospiraceae and as a consequence an increased level of butyric acid was observed. In addition, the administration of probiotics plus inulin modified the gut microbiota composition also at strain level since probiotics alone or in combination with inulin select specific Faecalibacterium prausnitzi strain populations. The metagenomic analysis showed in probiotic plus prebiotic fed broilers a higher number of genes required for branched-chain amino acid biosynthesis belonging to selected F. prausnitzi strains, which are crucial in increasing immune function resistance to pathogens. In the presence of the probiotic/prebiotic a reduction in the occurrence of antibiotic resistance genes belonging to aminoglycoside, beta-lactamase and lincosamide family was observed.

Conclusions: The positive microbiome modulation observed is particularly relevant, since the use of these alternative ingredients could promote a healthier status of the broiler's gut.

Keywords: Broiler chickens; Inulin; Lactic acid bacteria; Prebiotic; Probiotic; microbiota.