Evaluation of a novel Clostridioides difficile-selective growth broth under aerobic conditions

Yukitaka Ito; Naomi Kaneko; Mieko Yoshida; Haruo Nakayama

doi:10.1016/j.diagmicrobio.2023.116020

Evaluation of a novel Clostridioides difficile-selective growth broth under aerobic conditions

Diagn Microbiol Infect Dis. 2023 Oct;107(2):116020. doi: 10.1016/j.diagmicrobio.2023.116020. Epub 2023 Jul 11.

Authors

Yukitaka Ito¹, Naomi Kaneko², Mieko Yoshida², Haruo Nakayama³

Affiliations

¹ Division of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Meguro, Tokyo, Japan; Department of Infection and prevention, Toho University Ohashi Medical Center, Meguro, Tokyo, Japan. Electronic address: ito-yuki4121@med.toho-u.ac.jp.
² Division of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Meguro, Tokyo, Japan.
³ Division of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Meguro, Tokyo, Japan; Department of Infection and prevention, Toho University Ohashi Medical Center, Meguro, Tokyo, Japan.

PMID: 37536259
DOI: 10.1016/j.diagmicrobio.2023.116020

Abstract

A newly developed Clostridioides difficile-selective growth broth, which can be cultured under aerobic conditions, was found to have a sensitivity/specificity (98%/89%) comparable to conventional anaerobic culture methods. This might be a powerful tool for diagnosing Clostridioides difficile infection in resource-limited regions and health care settings in the future.

Keywords: Aerobic culture; Anaerobe; C. difficile-selective growth broth; Clostridioides difficile.

MeSH terms

Bacteriological Techniques / methods
Clostridioides
Clostridioides difficile*
Clostridium Infections* / diagnosis
Humans
Sensitivity and Specificity