Proteomic profiling of purified avian leukosis virus subgroup J particles

Sheng Chen; Yiming Yan; Liguo Gao; Shuang Gao; Keyu Feng; Hongxin Li; Xinheng Zhang; Weiguo Chen; Feng Chen; Qingmei Xie

doi:10.1016/j.vetmic.2023.109821

Proteomic profiling of purified avian leukosis virus subgroup J particles

Vet Microbiol. 2023 Sep:284:109821. doi: 10.1016/j.vetmic.2023.109821. Epub 2023 Jun 22.

Authors

Sheng Chen¹, Yiming Yan², Liguo Gao³, Shuang Gao², Keyu Feng⁴, Hongxin Li⁴, Xinheng Zhang⁴, Weiguo Chen⁴, Feng Chen⁵, Qingmei Xie⁶

Affiliations

¹ Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, Guangdong, PR China.
² Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 510642, PR China.
³ Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
⁴ Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China.
⁵ South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, Guangdong, PR China.
⁶ Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, Guangdong, PR China. Electronic address: qmx@scau.edu.cn.

PMID: 37536160
DOI: 10.1016/j.vetmic.2023.109821

Abstract

While the presence of host cell proteins in virions and their role in viral life cycles have been demonstrated in various viruses, such characteristics have remained largely unknown in avian leukosis virus (ALV). To investigate whether this is the case in ALV, we purified high-integrity and high-purity virions from the avian leukosis virus subgroup J (ALV-J) and subjected them to proteome analysis using nano LC-MS/MS. This analysis identified 53 cellular proteins that are incorporated into mature ALV-J virions, and we verified the reliability of the packaged cellular proteins through subtilisin digestion and immunoblot analysis. Functional annotation revealed the potential functions of these proteins in the viral life cycle and tumorigenesis. Overall, our findings have important implications for understanding the interaction between ALV-J and its host, and provide new insights into the cellular requirements that define ALV-J infection.

Keywords: Avian leukosis virus subgroup J; LC-MS/MS; Subtilisin; Virion proteomic; Virus purification.

MeSH terms

Animals
Avian Leukosis Virus* / genetics
Avian Leukosis*
Chickens
Proteomics
Reproducibility of Results
Tandem Mass Spectrometry / veterinary