Conifer-like TiSnO2 nanorods mixed metal oxide was synthesized via the one-pot polyol method utilizing ethylene glycol (EG), poly(diallyldimethylammonium chloride) (PDDA), tin(II) chloride dihydrate (SnCl2·2H2O), and titanium(IV)-ethylhexanoate (TE) as precursor materials, aimed at room temperature H2S gas sensing. The effects of polyol duration time and capping agent concentration (PDDA) were examined to explore the morphological, structural, and gas-sensing characteristics, as well as to propose potential growth mechanisms of conifer-like TiSnO2 nanorods mixed metal oxide. The morphology and composition of the synthesized TiSnO2 mixed metal oxide were carried out employing scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffractometry (XRD). The experimental findings demonstrated a significant influence of polyol duration time and PDDA concentration on the morphological evolution of the synthesized TiSnO2 mixed metal oxide structures. Comparative gas-sensing analysis indicated that the conifer-like TiSnO2 nanorods mixed metal oxide exhibited the highest response (2.45%) towards H2S gas at a concentration of 1 ppm, along with a low detection limit (0.20 ppm) and good linearity (R2 = 0.9865) within the range of 1-15 ppm of H2S gas at room temperature.