Promotion of skin wound healing using hypoimmunogenic epidermal cell sheets

Hongqing Zhao; Jiachen Sun; Yating Wu; Junbo Zhang; Chuan'an Shen

doi:10.1016/j.reth.2023.07.003

Promotion of skin wound healing using hypoimmunogenic epidermal cell sheets

Regen Ther. 2023 Jul 20:24:245-255. doi: 10.1016/j.reth.2023.07.003. eCollection 2023 Dec.

Authors

Hongqing Zhao^{1

2}, Jiachen Sun², Yating Wu², Junbo Zhang², Chuan'an Shen³

Affiliations

¹ Nanbu County People's Hospital, Nanchong City, Sichuan Province, 637300, China.
² Jinzhou Medical University, No.82 Songpo Road, Guta District, Jinzhou 121001, Liaoning Province, China.
³ Department of Burn Surgery, Fourth Medical Center, PLA General Hospital, No 51 Fucheng Road, Haidian District, Beijing 100089, China.

Abstract

Objective: The physiological process of wound healing is dynamic, continuous, and intricate. Nowadays, full-thickness burn wounds are treated by autologous skin transplantation. Unfortunately, when substantial burns develop, there are fewer donor sites accessible, making it difficult to satisfy the requirement for large-scale skin transplants and increasing the risk of patient mortality. This study investigated the possibility of using a newly created hypoimmunogenic epidermal cell sheet to heal skin wounds.

Methods: Transfection with lentivirus was used to generate Keratinocytes (KCs) that overexpress Indoleamine 2,3-Dioxygenase (IDO). Western blotting and quantitative polymerase chain reaction were used to measure IDO levels. To evaluate the function of IDO⁺ keratinocytes, CCK-8 and Transwell assays were performed. In cell sheet induction media, KCs and Fibroblasts (FBs) were cultured to yield epidermal cell sheets. The full-thickness skin excisions of BALB/c mice were transplanted with epidermal cell sheets. To assess the tumorigenicity of IDO⁺ keratinocytes, BALB/c nude mouse xenograft models were also used. CD3 and CD31 immunofluorescence labeling of wound tissue on day 12 to identify T lymphocyte infiltration and capillary development. ELISA measurement of IL-1 and TNF-α concentrations.

Results: IDO ⁺ keratinocytes dramatically enhanced the expression levels of IDO mRNA and protein, as well as the amount of kynurenine in the conditioned media of IDO⁺ keratinocytes, compared to the Control and NC groups. CD8⁺ T cell apoptosis was considerably greater in the IDO group than in the Control and NC groups. Nevertheless, the proliferation and migratory capabilities of IDO⁺ keratinocytes were not substantially different from those of the Control and NC groups. In vitro cultivation of the hypoimmunogenic epidermal cell sheet was effective. In vivo transplantation experiments demonstrated that IDO⁺ epidermal cell sheets can effectively promote wound healing without tumorigenicity, and IDO⁺ epidermal cell sheets may promote wound healing by decreasing the expression levels of inflammatory factors (TNF and IL-1) in wound tissue, decreasing CD3⁺ T lymphocytes, and increasing infiltration and new capillaries in wound tissue.

Conclusion: In this study, we successfully constructed the hypoimmunogenic epidermal cell sheet and demonstrated that the hypoimmunogenic epidermal cell sheet could accelerate wound healing.

Keywords: Allogenic skin substitute; Epidermal cell sheet; Indoleamine 2,3-dioxygenase; Skin; Wound healing.