Single-cell immune profiling reveals broad anti-inflammation response in bipolar disorder patients with quetiapine and valproate treatment

Lingbin Qi; Yan Qiu; Sujuan Li; Ning Yi; Chanyi Li; Ziwei Teng; Shiping Li; Xuelei Xu; Bin Lang; Jindong Chen; Chunbing Zheng; Yuan Yang; Jiangzhou Hua; Cheng Wang; Haishan Wu; Zhigang Xue; Bo Lv

doi:10.1016/j.isci.2023.107057

Single-cell immune profiling reveals broad anti-inflammation response in bipolar disorder patients with quetiapine and valproate treatment

iScience. 2023 Jun 7;26(7):107057. doi: 10.1016/j.isci.2023.107057. eCollection 2023 Jul 21.

Authors

Lingbin Qi¹, Yan Qiu², Sujuan Li², Ning Yi¹, Chanyi Li¹, Ziwei Teng², Shiping Li³, Xuelei Xu², Bin Lang², Jindong Chen^{2

4}, Chunbing Zheng³, Yuan Yang³, Jiangzhou Hua³, Cheng Wang³, Haishan Wu², Zhigang Xue¹, Bo Lv¹

Affiliations

¹ Department of Regenerative Medicine, Translational Center for Stem Cell Research, Tongji Hospital, Tongji University School of Medicine, Shanghai 200092, China.
² Department of Psychiatry, and National Clinical Research Center for Mental Disorders, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
³ Stem Cell and Regenerative Medicine Engineering Research Center of Hunan Province, Hunan Yuanpin Cell Technology Co. Ltd, 102 Dongwu Road, Changsha City, Hunan Province 410100, China.
⁴ Department of Psychiatry, Xiangya Boai Rehabilitation Hospital, Changsha 410100, China.

Abstract

Bipolar disorder (BD) is a common mental disorder characterized by manic and depressive episodes. Mood disorders have been associated with immune dysfunction. The combination of quetiapine and valproate has shown positive effects in treating BD, but the impact on immune dynamics remains less understood. Using single-cell RNA sequencing, we observed that B cells exhibited downregulation of inflammation-related genes, while pro-inflammatory mast and eosinophil cells decreased following treatment. Ribosomal peptide production genes were found to be reduced in both B and T cells after treatment. Additionally, our findings suggest that the combined therapy effectively alleviates inflammation by reducing myloid-mediated immune signaling pathways. This study provides valuable insights into the immune atlas and uncovers a potential mechanism for immune disorder alleviation in patients with BD treated with quetiapine and valproate.

Keywords: Biological sciences; Immunology; Omics; Transcriptomics.