Evolutional insights into the interaction between Rab7 and RILP in lysosome motility

Gaofeng Cui; Zhiyan Jiang; Yaoyao Chen; Yun Li; Shupei Ai; Ranran Sun; Xin Yi; Guohua Zhong

doi:10.1016/j.isci.2023.107040

Evolutional insights into the interaction between Rab7 and RILP in lysosome motility

iScience. 2023 Jun 7;26(7):107040. doi: 10.1016/j.isci.2023.107040. eCollection 2023 Jul 21.

Authors

Gaofeng Cui^{1

2}, Zhiyan Jiang¹, Yaoyao Chen¹, Yun Li¹, Shupei Ai¹, Ranran Sun¹, Xin Yi¹, Guohua Zhong¹

Affiliations

¹ College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
² Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of Forestry, Guangzhou 510520, China.

Abstract

Lysosome motility is critical for the cellular function. However, Rab7-related transport elements showed genetic differences between vertebrates and invertebrates, making the mechanism of lysosomal motility mysterious. We suggested that Rab7 interacted with RILP as a feature of highly evolved organisms since they could interact with each other in Spodoptera frugiperda but not in Drosophila melanogaster. The N-terminus of Sf-RILP was identified to be necessary for their interaction, and Glu61 was supposed to be the key point for the stability of the interaction. A GC-rich domain on the C-terminal parts of Sf-RILP hampered the expression of Sf-RILP and its interaction with Sf-Rab7. Although the corresponding vital amino acids in the mammalian model at the C-terminus of Sf-RILP turned to be neutral, the C-terminus would also help with the homologous interactions between RILP fragments in insects. The significantly different interactions in invertebrates shed light on the biodiversity and complexity of lysosomal motility.

Keywords: Biochemistry; Entomology; Evolutionary biology.